Whole-organism cellular gene-expression atlas reveals conserved cell types in the ventral nerve cord of Platynereis dumerilii
| Autor: | Kaia Achim, Detlev Arendt, Paola Bertucci, Hernando Martínez Vergara, Péter Hantz, Pavel Vopalensky, Maria Antonietta Tosches |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell type Interneuron Cellular differentiation Biology Sackler Colloquium on Gene Regulatory Networks and Network Models in Development and Evolution Models Biological 03 medical and health sciences biology.animal medicine Animals Gene Body Patterning Neurons Multidisciplinary Gene Expression Profiling Neural tube Vertebrate Gene Expression Regulation Developmental Cell Differentiation Polychaeta Anatomy Biological Evolution Multicellular organism 030104 developmental biology medicine.anatomical_structure Evolutionary biology Ventral nerve cord Algorithms |
| Popis: | The comparative study of cell types is a powerful approach toward deciphering animal evolution. To avoid selection biases, however, comparisons ideally involve all cell types present in a multicellular organism. Here, we use image registration and a newly developed “Profiling by Signal Probability Mapping” algorithm to generate a cellular resolution 3D expression atlas for an entire animal. We investigate three-segmented young worms of the marine annelid Platynereis dumerilii , with a rich diversity of differentiated cells present in relatively low number. Starting from whole-mount expression images for close to 100 neural specification and differentiation genes, our atlas identifies and molecularly characterizes 605 bilateral pairs of neurons at specific locations in the ventral nerve cord. Among these pairs, we identify sets of neurons expressing similar combinations of transcription factors, located at spatially coherent anterior-posterior, dorsal-ventral, and medial-lateral coordinates that we interpret as cell types. Comparison with motor and interneuron types in the vertebrate neural tube indicates conserved combinations, for example, of cell types cospecified by Gata1/2/3 and Tal transcription factors. These include V2b interneurons and the central spinal fluid-contacting Kolmer-Agduhr cells in the vertebrates, and several neuron types in the intermediate ventral ganglionic mass in the annelid. We propose that Kolmer-Agduhr cell-like mechanosensory neurons formed part of the mucociliary sole in protostome-deuterostome ancestors and diversified independently into several neuron types in annelid and vertebrate descendants. |
| Databáze: | OpenAIRE |
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