Heteromers of glutamate decarboxylase isoforms occur in rat cerebellum
| Autor: | Sabina N. Sheikh, David L. Martin |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gene isoform
endocrine system Carboxy-lyases endocrine system diseases Protein subunit Glutamate decarboxylase Molecular Sequence Data Enzyme-Linked Immunosorbent Assay Biology Biochemistry Cellular and Molecular Neuroscience Affinity chromatography Chinchilla Cerebellum Animals Amino Acid Sequence Rats Wistar Gel electrophoresis chemistry.chemical_classification Antiserum Glutamate Decarboxylase Immune Sera nutritional and metabolic diseases Molecular biology Amino acid Rats Isoenzymes Molecular Weight chemistry Peptides |
| Zdroj: | Journal of neurochemistry. 66(5) |
| ISSN: | 0022-3042 |
| Popis: | The subunit structure of brain glutamate decarboxylase in cerebellum was investigated by using gel electrophoresis and antisera that specifically recognize the individual isoforms of brain glutamate decarboxylase (termed GAD 65 and GAD 67 ). The antisera were prepared against peptides that corresponded to amino acid sequences specific to each isoform. Each antiserum reacted specifically with the appropriate peptide in an ELISA and with the appropriate form of GAD on immunoblots. Nondenaturing gradient gel electrophoresis indicated that GAD is principally multimeric with monomeric forms comprising |
| Databáze: | OpenAIRE |
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