Molecular Identification of Campylobacter concisus
| Autor: | M. I. Matsheka, A. J. Lastovica, B. Gay Elisha |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
DNA Bacterial Microbiology (medical) Molecular Sequence Data Campylobacter concisus Deoxyribonuclease HindIII HindIII medicine.disease_cause Polymerase Chain Reaction Campylobacter jejuni Restriction fragment Open Reading Frames Species Specificity Campylobacter Infections medicine Humans Genomic library Cloning Molecular ORFS Child Genetics biology Campylobacter Nucleic Acid Hybridization Bacteriology Sequence Analysis DNA Amplicon biology.organism_classification Molecular biology Bacterial Typing Techniques biology.protein DNA Probes |
| Zdroj: | Journal of Clinical Microbiology. 39:3684-3689 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.39.10.3684-3689.2001 |
| Popis: | A 1.6-kb DNA fragment isolated from a Campylobacter concisus genomic library gave C. concisus -specific restriction fragment length patterns when it was used as a probe in hybridization studies. All of the strains tested, including type strains and clinical isolates, contained a 0.5-kb Hin dIII fragment that hybridized to the probe. DNA sequencing of the 1.6-kb fragment identified three open reading frames (ORFs). One of the ORFs encodes the carboxy terminus of GyrB, and the translational products of ORF2 and ORF3 showed similarity to hypothetical proteins, previously identified in Campylobacter jejuni . DNA-DNA hybridization studies with a fragment internal to ORF3 showed that this sequence was responsible for the signal observed with the 0.5-kb Hin dIII fragment. A rapid PCR assay was developed and evaluated. Primers that annealed to the extremities of the 1.6-kb fragment were used to obtain an amplicon of the correct size from both reference and clinical strains of C. concisus . |
| Databáze: | OpenAIRE |
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