Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains
| Autor: | José L. Revuelta, David Díaz-Fernández, Tatiana Quinta Aguiar, Aloia Romaní, Victoria Isabel Martín, Rui Silva, Lucília Domingues, Alberto Jiménez |
|---|---|
| Přispěvatelé: | Universidade do Minho |
| Rok vydání: | 2019 |
| Předmět: |
Glycerol
0106 biological sciences Environmental Engineering Industrial Waste Bioengineering 010501 environmental sciences Xylose Eremothecium 01 natural sciences Hydrolysate Metabolic engineering chemistry.chemical_compound Biosynthesis 010608 biotechnology Ashbya gossypii Microbial lipids Molasses Food science Waste Management and Disposal 0105 earth and related environmental sciences 2. Zero hunger chemistry.chemical_classification Science & Technology Strain (chemistry) Renewable Energy Sustainability and the Environment General Medicine Lipids 6. Clean water Pyruvate carboxylase Enzyme Metabolic Engineering chemistry 13. Climate action Lignocellulosic hydrolysates Regulation |
| Zdroj: | GREDOS. Repositorio Institucional de la Universidad de Salamanca instname Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2019.122054 |
| Popis: | Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054. This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks. This work was supported by: the Spanish Ministerio de Economía y Competitividad (grant numbers BIO2014-56930-PandBIO2017-88435R) and the Junta de Castilla y León (grant number SA016P17); the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019, PhD grant PD/ BD/113812/2015 to R. Silva (Doctoral Program in Applied and Environmental Microbiology), the MultiBiorefinery project (POCI-010145-FEDER-016403) and the BioTecNorte operation (NORTE-010145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. DDF was recipient of USAL predoctoral fellowship from the University of Salamanca. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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