Macrocystis pyriferasource of nutrients for the production of carotenoids by a marine yeastRhodotorula mucilaginosa

Autor: Liset Flores, M. E. Lienqueo, Allison Leyton, Päivi Mäki-Arvela, Carolina Shene
Rok vydání: 2019
Předmět:
Zdroj: Journal of Applied Microbiology. 127:1069-1079
ISSN: 1365-2672
1364-5072
Popis: Aims To evaluate an aqueous extract of Macrocystis pyrifera as a nutrient source for the production of carotenoids by a marine Rhodotorula mucilaginosa isolated from seaweed samples. Materials and results The effect of different culture conditions on the concentration of biomass and total pigments was evaluated using a Box-Behnken experimental design. The seaweed extract contained 15% w w-1 of protein and 20% w w-1 of carbohydrate; the main sugar in this fraction was trehalose (78%). The culture conditions that maximize the total pigment concentration (1·84 ± 0·03 mg l-1 ) were initial pH equal to 7, yeast extract as nitrogen source at a concentration of 4 g l-1 , seaweed extract concentration at 25% v v-1 , incubation performed at 25°C and 150 rev min-1 during 6 days. Under optimal growth conditions, three carotenoids were identified among the pigments produced by R. mucilaginosa, lycopene (38·4 ± 9·4%), β-carotene (21·8 ± 1·5%) and astaxanthin (1·8 ± 0·3%). Conclusions Carotenoids of commercial interest (lycopene, β-carotene and astaxanthin) can be produced using a marine R. mucilaginosa cultivated with an aqueous extract of M. pyrifera as nutrient source. The total pigment concentration in the culture ranged between 0·82 and 1·84 mg l-1 , and was significantly affected by the concentration of the seaweed extract, and yeast extract. Significance and impact of the study This work demonstrates that M. pyrifera can be used as a nutrient source for the production of carotenoids by the marine yeast.
Databáze: OpenAIRE
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