HCV genotype determination in monoinfected and HIV co-infected patients in Cuba
| Autor: | Susel Sariego Frómeta, Licel de los Ángeles Rodríguez Lay, Milay Bello Núñez, Hermes Pedreira da Silva Filho, María Caridad Montalvo Villalba, Mitermayer G. Reis, Judith M. Hübschen, Meilin Sánchez Wong, Santiago Dueñas Carrera, Marité Bello Corredor, Marcia Samada, Lidunka Valdés Alonso, Jeny Marante Hernández |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Adolescent Genotype Hepatitis C virus HIV Infections Hepacivirus Biology medicine.disease_cause Polymerase Chain Reaction law.invention Men who have sex with men Young Adult law medicine Prevalence Humans Homosexuality Male Child Polymerase chain reaction Phylogeny Aged DNA Primers Molecular Epidemiology Molecular epidemiology Unsafe Sex Coinfection Public Health Environmental and Occupational Health virus diseases Cuba General Medicine Hepatitis C Middle Aged medicine.disease Virology Infectious Diseases RNA Viral Parasitology Female Restriction fragment length polymorphism Sequence Analysis |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 106(12) |
| ISSN: | 1878-3503 |
| Popis: | With the aim to characterize the HCV genotype distribution in Cuba, sera were collected from two subgroups: HCV-monoinfected and HCV/HIV co-infected patients. A combination of reverse transcription-PCR using genotype-specific primers, restriction fragment length polymorphism and sequencing was used to determine the genotype of 84 samples. Seventy-nine (94%) showed single infections (10 [12%] were genotype 1a and 69 [82%] genotype 1b) and 5 (6%) samples corresponded to mixed infections (2 [2%] with genotypes 1a/3a and 1 sample [1%] each with 1b/3a, 1b/4a and 1a/1b/3a). HCV/HIV co-infected subjects had a higher frequency of mixed infections (p=0.08), infection with genotype 3a (p=0.18) and for the first time genotype 4a was found. There was no association of any demographic characteristics with any specific genotype although HCV/HIV co-infected patients showed a tendency to have mixed genotypes in those older than 45 years of age (p=0.11). Phylogenetic analysis showed that HCV isolates clustered with subtypes 1b (n=15, maximal genetic distance 2.51%) and 1a (n=2, maximal genetic distance 0.35%). This report presents the prevalence of HCV genotypes in monoinfected and HIV co-infected patients, mixed HCV infections in HCV/HIV co-infected men who have sex with men with high-risk sexual practices and for the first time identifies that the uncommon genotype 4a can be present in a patient co-infected with HIV. |
| Databáze: | OpenAIRE |
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