Single molecule atomic force microscopy studies of photosensitized singlet oxygen behavior on a DNA origami template
| Autor: | Larisa Kovbasyuk, Sarah Helmig, Jørgen Kjems, Kurt V. Gothelf, Flemming Besenbacher, Jacob Arnbjerg, Peter R. Ogilby, Alexandru Rotaru, Dumitru Arian, Andriy Mokhir |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular photosensitizer General Physics and Astronomy Nanotechnology Conjugated system Microscopy Atomic Force singlet oxygen chemistry.chemical_compound DNA origami Molecule General Materials Science A-DNA Photosensitizing Agents Singlet Oxygen Singlet oxygen Oligonucleotide General Engineering DNA Single Molecule Imaging Nanostructures Folding (chemistry) chemistry single molecule imaging Nucleic Acid Conformation AFM |
| Zdroj: | Helmig, S W, Rotaru, A, Arian, D, Kovbasyuk, L, Arnbjerg, J, Ogilby, P R, Kjems, J, Mokhir, A, Besenbacher, F & Gothelf, K V 2010, ' Single Molecule Atomic Force Microscopy Studies of Photosensitized Singlet Oxygen Behavior on a DNA Origami Template ', A C S Nano, vol. 4, no. 12, pp. 7475-7480 . https://doi.org/10.1021/nn102701f |
| ISSN: | 1936-086X |
| DOI: | 10.1021/nn102701f |
| Popis: | DNA origami, the folding of a long single-stranded DNA sequence (scaffold strand) by hundreds of short synthetic oligonucleotides (staple strands) into parallel aligned helices, is a highly efficient method to form advanced self-assembled DNA-architectures. Since molecules and various materials can be conjugated to each of the short staple strands, the origami method offers a unique possibility of arranging molecules and materials in well-defined positions on a structured surface. Here we combine the action of light with AFM and DNA nanostructures to study the production of singlet oxygen from a single photosensitizer molecule conjugated to a selected DNA origami staple strand on an origami structure. We demonstrate a distance-dependent oxidation of organic moieties incorporated in specific positions on DNA origami by singlet oxygen produced from a single photosensitizer located at the center of each origami. |
| Databáze: | OpenAIRE |
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