Epitope mapping of pneumococcal surface protein A of strain Rx1 using monoclonal antibodies and molecular structure modelling
| Autor: | Audun Aase, Gunnhild Rødal, Jan Kolberg, James E. Littlejohn, Mark J. Jedrzejas |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Models Molecular medicine.drug_class Immunology Enzyme-Linked Immunosorbent Assay Monoclonal antibody Microbiology Epitope Pneumococcal Infections law.invention Pneumococcal Vaccines Antigen Bacterial Proteins law medicine Immunology and Allergy Humans biology Antibodies Monoclonal General Medicine medicine.disease Flow Cytometry Virology Recombinant Proteins Pneumococcal infections Infectious Diseases Epitope mapping Streptococcus pneumoniae Pneumococcal vaccine biology.protein Recombinant DNA Electrophoresis Polyacrylamide Gel Antibody Epitope Mapping |
| Zdroj: | FEMS immunology and medical microbiology. 39(3) |
| ISSN: | 0928-8244 |
| Popis: | Pneumococcal surface protein A (PspA) is an antigenic variable vaccine candidate of Streptococcus pneumoniae. Epitope similarities between PspA from the American vaccine candidate strain Rx1 and Norwegian clinical isolates were studied using PspA specific monoclonal antibodies (mAbs) made against clinical Norwegian strains. Using recombinant PspA/Rx1 fragments and immunoblotting the epitopes for mAbs were mapped to two regions of amino acids, 1–67 and 67–236. The discovered epitopes were visualized by modelling of the PspA:Fab part of mAb in three dimensions. Flow cytometric analysis showed that the epitopes for majority of mAbs were accessible for antibody binding on live pneumococci. Also, the epitopes for majority of the mAbs are widely expressed among clinical Norwegian isolates. |
| Databáze: | OpenAIRE |
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