Berberine alters gut microbial function through modulation of bile acids
Autor: | Lindsey Ly, Jason M. Ridlon, George E. Chlipala, H. Rex Gaskins, Hiroshi Nittono, Hajime Takei, Saravanan Devendran, Tsuyoshi Murai, Vance J. McCracken, Takao Kurosawa, Stefan J. Green, Patrick M. Gillevet, Tyler Moore, Purna C. Kashyap, Genta Kakiyama, Heidi L. Doden, Patricia G. Wolf |
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Rok vydání: | 2020 |
Předmět: |
Microbiology (medical)
DNA Bacterial Male Berberine medicine.drug_class lcsh:QR1-502 Carbohydrate metabolism Pharmacology Biology Microbiology DNA Ribosomal lcsh:Microbiology Bile Acids and Salts 03 medical and health sciences chemistry.chemical_compound Mice Bacterial Proteins Species Specificity RNA Ribosomal 16S medicine Animals Metabolomics Microbiome RNA-Seq Gut bacteria 030304 developmental biology 0303 health sciences Gastrointestinal tract Bile acid Bacteria 030306 microbiology Cholesterol Sequence Analysis RNA Gene Expression Profiling Deoxycholic acid Gnotobiotic mice Gene Expression Regulation Bacterial biology.organism_classification Bile acids Gastrointestinal Microbiome chemistry Female Network analysis Nutraceutical Research Article |
Zdroj: | BMC Microbiology, Vol 21, Iss 1, Pp 1-15 (2021) BMC Microbiology |
Popis: | Background Berberine (BBR) is a plant-based nutraceutical that has been used for millennia to treat diarrheal infections and in contemporary medicine to improve patient lipid profiles. Reduction in lipids, particularly cholesterol, is achieved partly through up-regulation of bile acid synthesis and excretion into the gastrointestinal tract (GI). The efficacy of BBR is also thought to be dependent on structural and functional alterations of the gut microbiome. However, knowledge of the effects of BBR on gut microbiome communities is currently lacking. Distinguishing indirect effects of BBR on bacteria through altered bile acid profiles is particularly important in understanding how dietary nutraceuticals alter the microbiome. Results Germfree mice were colonized with a defined minimal gut bacterial consortium capable of functional bile acid metabolism (Bacteroides vulgatus, Bacteroides uniformis, Parabacteroides distasonis, Bilophila wadsworthia, Clostridium hylemonae, Clostridium hiranonis, Blautia producta; B4PC2). Multi-omics (bile acid metabolomics, 16S rDNA sequencing, cecal metatranscriptomics) were performed in order to provide a simple in vivo model from which to identify network-based correlations between bile acids and bacterial transcripts in the presence and absence of dietary BBR. Significant alterations in network topology and connectivity in function were observed, despite similarity in gut microbial alpha diversity (P = 0.30) and beta-diversity (P = 0.123) between control and BBR treatment. BBR increased cecal bile acid concentrations, (P < 0.05), most notably deoxycholic acid (DCA) (P +/H+ antiporter, cell wall synthesis/repair, carbohydrate metabolism and amino acid metabolism. Bile acid concentrations in the GI tract increased significantly during BBR treatment and developed extensive correlation networks with expressed genes in the B4PC2 community. Conclusions This work has important implications for interpreting the effects of BBR on structure and function of the complex gut microbiome, which may lead to targeted pharmaceutical interventions aimed to achieve the positive physiological effects previously observed with BBR supplementation. |
Databáze: | OpenAIRE |
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