Circulating miR-29c, miR-30c, miR-193a-5p and miR-885-5p: Novel potential biomarkers for HTLV-1 infection diagnosis
| Autor: | Haidar Akl, Hussein Fayyad-Kazan, Rania El Majzoub, Rim ElDirani, Mohammad Fayyad-Kazan, Bassam Badran, Eva Hamade, Nizar Bitar |
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| Rok vydání: | 2019 |
| Předmět: |
Genetic Markers
Male 0301 basic medicine Microbiology (medical) Oncology medicine.medical_specialty 030106 microbiology Biology Microbiology 03 medical and health sciences Mir 193a 3p Internal medicine microRNA Genetics medicine Humans Molecular Biology Ecology Evolution Behavior and Systematics Oligonucleotide Array Sequence Analysis Human T-lymphotropic virus 1 Gene Expression Profiling Curve analysis medicine.disease HTLV-I Infections MicroRNAs Leukemia Early Diagnosis 030104 developmental biology Infectious Diseases Virus type Case-Control Studies Potential biomarkers Biomarker (medicine) Female HTLV-1 Infection |
| Zdroj: | Infection, Genetics and Evolution. 74:103938 |
| ISSN: | 1567-1348 |
| Popis: | Human T-cell leukemia virus type 1 (HTLV-1) is an oncoretrovirus that infects 5–10 million people worldwide. Currently, different methods are used to test HTLV-1 infection. However, a biomarker that could enable an early and accurate diagnosis of HTLV-1 infection is still lacking. Here, we compared the serum miRNA expression profile in HTLV-1 infected patients versus healthy individuals to identify a potential biomarker for diagnosis of HTLV-1 infection.TaqMan miRNA microarray (TLDA) was carried out to compare the miRNA expression profile in infected versus healthy individuals. Quantitative real-time RT-PCR (qRT-PCR) was applied to validate TLDA results. Receiver-operator characteristic (ROC) curve analysis was performed to determine the diagnostic accuracy of the most highly and significantly identified deregulated miRNA(s) as potential biomarker(s). We identified deregulated expression for ten miRNAs with miR-127, miR-136, miR-142-3p, miR-221, and miR-423-5p being down-regulated whilst let-7b, miR-29c, miR-30c, miR-193a-5p, and miR-885-5p being up-regulated in infected individuals. ROC curve analyses showed an AUC ( A reas U nder the ROC C urve) of 0.875 (95% CI: 0.7819–0.9581; P = .0021), 0.861 (95% CI: 0.7596–0.9754; P = .003), 0.856 (95% CI: 0.689–0.895; P = .011), and 0.849 (95% CI: 0.678–0.855; P = .017) for miR-29c, miR-30c, miR-193a-5p, and miR-885-5p respectively. Combined ROC analyses using these 4 miRNAs showed a greater AUC of 0.907 (95% CI: 0.809–1; P = .000001) indicating a robust diagnostic value of these 4 miRNAs. Our findings highlight serum miR-29c, miR-30c, miR-193a-5p and miR-885-5p as novel potential biomarkers important for HTLV-1 diagnosis. |
| Databáze: | OpenAIRE |
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