DNA Fragmentation in Central Nervous System Vascular Malformations
| Autor: | Yasushi Takagi, Nobuo Hashimoto, Kazuhiko Nozaki, Itaro Hattori, M. Ishikawa |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Intracranial Arteriovenous Malformations Male Hemangioma Cavernous Central Nervous System Pathology medicine.medical_specialty Adolescent Endothelium Central nervous system Apoptosis DNA Fragmentation Hemangioma In Situ Nick-End Labeling Humans Medicine Fragmentation (cell biology) Central Nervous System Vascular Malformations Enzyme Precursors Brain Neoplasms Caspase 3 business.industry Vascular disease Arteriovenous malformation Cerebral Arteries Middle Aged medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic medicine.anatomical_structure Caspases DNA fragmentation Female Surgery Neurology (clinical) business |
| Zdroj: | Acta Neurochirurgica. 142:987-994 |
| ISSN: | 0942-0940 0001-6268 |
| DOI: | 10.1007/s007010070053 |
| Popis: | Recent studies have shown that apoptosis plays an important role in vascular remodeling. We examined central nervous system vascular malformations for the presence of DNA fragmentation which is the evidence of apoptosis. We hypothesize that vascular remodeling through apoptosis may be responsible for recurrence or hemorrhage in these lesions. We examined the specimens of central nervous system vascular malformations by in situ end labeling (ISEL) of fragmented DNA. Moreover, we examined the expression of Caspase-3 which is apoptosis-related proteins in these lesions by immunohistochemistry. DNA fragmentation was observed in all 15 arteriovenous malformation (AVM) specimens. ISEL-positive cells were mainly distributed in the endothelium, media and perivascular tissue. In cavernous hemangioma (CH), DNA fragmentation was also observed in all 5 specimens. ISEL-positive cells were distributed in the endothelium, subendothelium and intercavernous matrix. Thirteen out of 15 AVM lesions stained positive for Caspase-3. Caspase-3 immunoreactivity was mainly distributed in the endothelium, media and perivascular tissue. This distribution was similar to that of ISEL positive cells. As for CHs, all 5 lesions stained positive for Caspase-3. Caspase-3 immunoreactivity was distributed in the endothelium, subendothelium and intercavernous matrix. Our findings indicate that apoptotic cell death and vascular remodeling play a role in the development and maintenance of vascular malformations. |
| Databáze: | OpenAIRE |
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