Generic gas chromatography-flame ionization detection method for quantitation of volatile amines in pharmaceutical drugs and synthetic intermediates
Autor: | Gabriel C. Graffius, Daniel Zewge, Holst M. Halsey, Robert Hartman, Brandon M. Jocher, Xiaodong Bu, Erik L. Regalado, Gary Lee, Frank Bernardoni |
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Rok vydání: | 2017 |
Předmět: |
Detection limit
Flame Ionization Chromatography Chromatography Gas Chemistry Chemistry Pharmaceutical 010401 analytical chemistry Organic Chemistry General Medicine Repeatability 010402 general chemistry 01 natural sciences Biochemistry Method development 0104 chemical sciences Analytical Chemistry law.invention Capillary column Pharmaceutical Preparations law Gas chromatography flame ionization detector Flame ionization detector Amine gas treating Gas chromatography Amines |
Zdroj: | Journal of chromatography. A. 1518 |
ISSN: | 1873-3778 |
Popis: | Volatile amines are among the most frequently used chemicals in organic and pharmaceutical chemistry. Synthetic route optimization often involves the evaluation of several different amines requiring the development and validation of analytical methods for quantitation of residual amine levels. Herein, a simple and fast generic GC-FID method on an Agilent J&W CP-Volamine capillary column (using either He or H2 as the carrier gas) capable of separating over 25 volatile amines and other basic polar species commonly used in pharmaceutical chemistry workflows is described. This 16min method is successfully applied to the analysis and quantitation of volatile amines in a variety of pharmaceutically-related drugs and synthetic intermediates. Method validation experiments showed excellent analytical performance in linearity, recovery, repeatability, and limit of quantitation and detection. In addition, diverse examples for the application of this method to the simultaneous determination of other amine-related chemicals in reaction mixtures are illustrated, thereby indicating that these GC-FID method conditions can be effectively used as starting point during method development for the analysis of other basic polar species beyond the validated list of amines described in this study. |
Databáze: | OpenAIRE |
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