Long noncoding RNA AC073284.4 suppresses epithelial-mesenchymal transition by sponging miR-18b-5p in paclitaxel-resistant breast cancer cells
| Autor: | Wenrui Wang, Changjie Chen, Yueyue Wang, Qingling Yang, Shuo Yang, Lei Yan, Sulian Chen, Henan Xu, Zheng‐Yuan Dong, Tiantian Chen |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Paclitaxel Physiology Clinical Biochemistry Cell Breast Neoplasms Biology Metastasis 03 medical and health sciences 0302 clinical medicine Cell Movement Cell Line Tumor medicine Humans Neoplasm Invasiveness Epithelial–mesenchymal transition Reporter gene Competing endogenous RNA GTPase-Activating Proteins Dock4 Cancer Cell Biology medicine.disease Long non-coding RNA Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology medicine.anatomical_structure Drug Resistance Neoplasm 030220 oncology & carcinogenesis Cancer research Female RNA Long Noncoding |
| Zdroj: | Journal of cellular physiology. 234(12) |
| ISSN: | 1097-4652 |
| Popis: | Breast cancer (BC) is the most prevalent malignant cancer in the world, is the leading cause of cancer-related death female. Recently, there is accumulating evidence that long noncoding RNAs (lncRNAs) might as an important role in the progression of BC. (epithelial-mesenchymal transition (EMT) is considered to play a vital role in tumor cells migration and invasion. Nevertheless, the entire biological mechanisms and functions of lncRNAs in tumor migration, invasion, and EMT remain uncertain. In the present research, we observed that the expression of lncRNA AC073284.4 was downregulated in BC paclitaxel-resistant (PR) cells (MCF-7/PR) and tissues. Bioinformatics analysis predicted that miR-18b-5p was a direct target of AC073284.4, which has been validated by dual-luciferase reporter gene assay. We further proved that AC073284.4 could directly bind to miR-18b-5p and relieve the suppression for dedicator of cytokinesis protein 4 (DOCK4). Furthermore, the underlying functional experiments demonstrated that AC073284.4 might sponge miR-18b-5p to attenuate the invasion, metastasis, and EMT of BC cell through upregulating DOCK4 expression. In summary, AC073284.4 might serve as a competing endogenous RNA (ceRNA) in BC progression via modulating miR-18b-5p/DOCK4 axis, which weakens EMT and migration of BC. These results suggesting that AC073284.4 might function as a potential novel diagnostic biomarker in the progression of BC. |
| Databáze: | OpenAIRE |
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