Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae
| Autor: | Kevin McEntee, Anthony P. Schmitt |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Hot Temperature
Saccharomyces cerevisiae Proteins Molecular Sequence Data Saccharomyces cerevisiae Oligonucleotides Biology DNA-binding protein Fungal Proteins Genes Reporter Transcription (biology) Northern blot Transcription factor Gene DNA Primers Zinc finger Reporter gene Multidisciplinary Base Sequence Zinc Fingers biology.organism_classification Molecular biology DNA-Binding Proteins Oxidative Stress Trans-Activators Research Article DNA Damage Protein Binding Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:5777-5782 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.12.5777 |
| Popis: | The stress response promoter element (STRE) confers increased transcription to a set of genes following environmental or metabolic stress in Saccharomyces cerevisiae. A lambda gt11 library was screened to isolate clones encoding STRE-binding proteins, and one such gene was identified as MSN2, which encoded a zinc-finger transcriptional activator. Disruption of the MSN2 gene abolished an STRE-binding activity in crude extracts as judged by both gel mobility-shift and Southwestern blot experiments, and overexpression of MSN2 intensified this binding activity. Northern blot analysis demonstrated that for the known or suspected STRE-regulated genes DDR2, CTT1, HSP12, and TPS2, transcript induction was impaired following heat shock or DNA damage treatment in the msn2-disrupted strain and was constitutively activated in a strain overexpressing MSN2. Furthermore, heat shock induction of a STRE-driven reporter gene was reduced more than 6-fold in the msn2 strain relative to wild-type cells. Taken together, these data indicate that Msn2p is the transcription factor that activates STRE-regulated genes in response to stress. Whereas nearly 85% of STRE-mediated heat shock induction was MSN2 dependent, there was significant MSN2-independent expression. We present evidence that the MSN2 homolog, MSN4, can partially replace MSN2 for transcriptional activation following stress. Moreover, our data provides evidence for the involvement of additional transcription factors in the yeast multistress response. |
| Databáze: | OpenAIRE |
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