Enantioseparation of cetirizine by sulfated-beta-cyclodextrin-mediated capillary electrophoresis
| Autor: | Yu-Wei Chou, Wei-Shan Huang, Chen-Chun Ko, Su-Hwei Chen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chromatography Time Factors Cyclodextrin Molecular Structure Chemistry Chemistry Pharmaceutical beta-Cyclodextrins Electrophoresis Capillary Filtration and Separation Stereoisomerism Dosage form Cetirizine Levocetirizine Analytical Chemistry Capillary electrophoresis medicine Enantiomer Enantiomeric excess medicine.drug |
| Zdroj: | Journal of separation science. 31(5) |
| ISSN: | 1615-9314 |
| Popis: | A sulfated beta-cyclodextrin (sulfated beta-CD)-mediated capillary electrophoresis method is described for the enantioseparation of cetirizine using achiral cefazolin as an internal standard. The enantioseparation of the drug was performed in a borate buffer (5 mM, pH 8.7) with 1% sulfated beta-CD (w/v) as chiral selector at 10 kV. Several parameters affecting the separation were studied, including the pH and the concentration of borate buffer and chiral selector. Under optimized conditions, a baseline separation of two enantiomers was achieved in less than 7 min. Using cefazolin as an internal standard (IS), the linear range of the method for the determination of levocetirizine was over 1.0 to 50.0 microg/mL; the detection limit (signal-to-noise ratio = 3) of levocetirizine was 0.5 microg/mL. The method allowed the enantioseparation of cetirizine in bulk samples and enantiomeric purity evaluation of levocetirizine (R-enantiomer) in pharmaceutical tablets (Xyzal), and it was also found to be suitable for enantioseparation in human plasma. |
| Databáze: | OpenAIRE |
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