Phytosphinganine Affects Plasmodesmata Permeability via Facilitating PDLP5-Stimulated Callose Accumulation in Arabidopsis
| Autor: | Xin Chen, Ning-Jing Liu, Bai-Hang Ju, Tao Zhang, Qiang-Hui Zhou, Yuan-Yuan Zhang, Zhao-Hui Liu, Hui-Shan Guo, Guozhu Li, Yong-Mei Qin, Yu-Xian Zhu |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Pseudomonas syringae Cell Communication Plant Science Plasmodesma 01 natural sciences Permeability 03 medical and health sciences chemistry.chemical_compound Sphingosine Plant Immunity Glucans Molecular Biology Pathogen Sphingolipids Cell Death biology Arabidopsis Proteins Callose Plasmodesmata Membrane Proteins food and beverages biology.organism_classification Sphingolipid Cell biology 030104 developmental biology chemistry Permeability (electromagnetism) Mutation Intracellular 010606 plant biology & botany |
| Zdroj: | Molecular Plant. 13:128-143 |
| ISSN: | 1674-2052 |
| DOI: | 10.1016/j.molp.2019.10.013 |
| Popis: | Plant plasmodesmata (PDs) are specialized channels that enable communication between neighboring cells. The intercellular permeability of PDs, which affects plant development, defense, and responses to stimuli, must be tightly regulated. However, the lipid compositions of PD membrane and their impact on PD permeability remain elusive. Here, we report that the Arabidopsis sld1 sld2 double mutant, lacking sphingolipid long-chain base 8 desaturases 1 and 2, displayed decreased PD permeability due to a significant increase in callose accumulation. PD-located protein 5 (PDLP5) was significantly enriched in the leaf epidermal cells of sld1 sld2 and showed specific binding affinity to phytosphinganine (t18:0), suggesting that the enrichment of t18:0-based sphingolipids in sld1 sld2 PDs might facilitate the recruitment of PDLP5 proteins to PDs. The sld1 sld2 double mutant seedlings showed enhanced resistance to the fungal-wilt pathogen Verticillium dahlia and the bacterium Pseudomonas syringae pv. tomato DC3000, which could be fully rescued in sld1 sld2 pdlp5 triple mutant. Taken together, these results indicate that phytosphinganine might regulate PD functions and cell-to-cell communication by modifying the level of PDLP5 in PD membranes. |
| Databáze: | OpenAIRE |
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