Detection of new allotypic variants of bovine λ-light chain constant regions in different cattle breeds
| Autor: | Claus-Peter Czerny, Dalia M. Aboelhassan, Ulrike S. Diesterbeck, Sina K. Stein |
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| Rok vydání: | 2012 |
| Předmět: |
Protein Conformation
Stereochemistry Molecular Sequence Data Immunology Cattle Diseases Breeding Immunoglobulin light chain Polymorphism Single Nucleotide 03 medical and health sciences 0302 clinical medicine Gene Frequency Species Specificity Animals Humans Genetic Predisposition to Disease Cloning Molecular Amino acid residue Immunoglobulin Allotypes 030304 developmental biology Genetics 0303 health sciences Constant region Base Sequence biology Isotype Allotype biology.protein Cattle Immunoglobulin Light Chains Antibody 030215 immunology Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 36:130-139 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2011.06.011 |
| Popis: | In the cattle breeds German Black Pied (GBP), German Simmental (GS), Holstein Friesian (HF), Aubrac (A) three transcribed allotypic variants in isotype IGLC2 and five allotypic variants in isotype IGLC3 were identified. Substitutions within the putative interface to CH1 at position 11 and 79 were noted. In IGLC2 b , K79E led to a charge conversion. In IGLC3 b and IGLC3 c , the E79N replacement removed the charge while the T11K substitution resulted in a positively charged amino acid residue. In addition, D15 and T16 were found in IGLC2 c , IGLC3 b , and IGLC3 c . Substitutions located on the outer site of the molecule were observed in IGLC2 b (V40, H45.5), IGLC2 c (A1, V40, D77), IGLC3 b (A1, D77, D109, P127), IGLC3 c (A1, G45.5, D77, D109, P127), IGLC3 d (D109), and IGLC3 e (A1). Amino acid residues P83 (IGLC2 c , IGLC3 b , IGLC3 c ), N93 (IGLC2 b ), D93 (IGLC3 b ), and G93 (IGLC3 c ) were positioned in cavities but seemed to be accessible for solvents. |
| Databáze: | OpenAIRE |
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