Weak multivalent biomolecular interactions: a strength versus numbers tug of war with implications for phase partitioning
| Autor: | Xavier Darzacq, Robert Tjian |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Transcription
Genetic Macromolecular Substances 1.1 Normal biological development and functioning Tug of war Context (language use) Molecular Dynamics Simulation Biology Genetic Underpinning research Live cell imaging Site occupancy Humans Molecular Biology Strong binding Biomolecular Condensates Regulation of gene expression Molecular interactions Binding Sites RNA-Binding Proteins Cell Compartmentation Molecular Imaging Eukaryotic Cells Gene Expression Regulation Perspective Biophysics RNA HIV/AIDS Generic health relevance Biochemistry and Cell Biology Transcription Function (biology) Protein Binding Developmental Biology |
| Zdroj: | RNA (New York, N.Y.), vol 28, iss 1 RNA |
| ISSN: | 1469-9001 1355-8382 |
| DOI: | 10.1261/rna.079004.121 |
| Popis: | In this short Perspective, we discuss how recent dynamic live-cell imaging experiments have challenged our understanding of mechanisms driving functional molecular interactions in vivo. While we have generally considered the formation of functional biomolecular complexes as resulting from the stable assembly of two or more partner molecules, here we entertain the possibility that function may actually be maintained while molecules are rapidly exchanged within a complex. We postulate that at high effective concentrations, even very weak interactions can lead to strong binding site occupancy and thereby mediate function in a highly dynamic fashion. This new perspective in our definition of what represents a functional complex in living cells and in vivo could significantly alter how we define the nature of molecular transactions critical for mediating regulation in the cellular context. These less conventional principles also allow a broadening of the mechanistic options we should explore when interpreting essential biological processes such as gene regulation. |
| Databáze: | OpenAIRE |
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