MOM-5 Frizzled regulates the distribution of DSH-2 to control C. elegans asymmetric neuroblast divisions
| Autor: | Gian Garriga, Gregory C. Ellis, Nancy C. Hawkins, Bruce Bowerman |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Frizzled
Cell division DSH-2 Dishevelled Proteins Cell Cycle Proteins Nervous System asymmetric cell division Animals Genetically Modified Dishevelled 03 medical and health sciences 0302 clinical medicine Neuroblast Cell cortex Asymmetric cell division Animals Caenorhabditis elegans Proteins Caenorhabditis elegans Molecular Biology DNA Primers 030304 developmental biology Neurons chemistry.chemical_classification 0303 health sciences biology Gene Expression Regulation Developmental Membrane Proteins Cell Biology biology.organism_classification Cell biology Microscopy Fluorescence chemistry RNA Interference MOM-5 Cell Division 030217 neurology & neurosurgery Signal Transduction Developmental Biology Asymmetric neuroblast division |
| Zdroj: | Developmental Biology. 284:246-259 |
| ISSN: | 0012-1606 |
| Popis: | Asymmetric cell divisions produce all 302 neurons of the C. elegans hermaphrodite. Here, we describe a role for a C. elegans Dishevelled homolog, DSH-2, in an asymmetric neuroblast division. In dsh-2 mutants, neurons normally descended from the anterior neuroblast daughter of the ABpl/rpppa blast cell were frequently duplicated, while non-neuronal cells produced by the posterior daughter cell were often missing. These observations indicate that in the absence of dsh-2 function, the posterior daughter cell was transformed into a second anterior-like cell. Loss of mom-5, a C. elegans frizzled homolog, produced a similar phenotype. We also show that the DSH-2 protein localized to the cell cortex in most cells of the embryo. In the absence of MOM-5/Fz, DSH-2 was localized to the cytoplasm, suggesting that MOM-5 regulates asymmetric cell division by controlling the localization of DSH-2. Although all neurons in C. elegans are produced by an invariant pattern of cell divisions, our results indicate that cell signaling may contribute to asymmetric neuroblast division during embryogenesis. |
| Databáze: | OpenAIRE |
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