Study protocol of the Bergen brain-gut-microbiota-axis study: A prospective case-report characterization and dietary intervention study to evaluate the effects of microbiota alterations on cognition and anatomical and functional brain connectivity in patients with irritable bowel syndrome
| Autor: | Eline Margrete Randulff Hillestad, Alfonso Benítez-Páez, Yolanda Sanz, Ingeborg Brønstad, Katarina Lundervold, Jan Gunnar Hatlebakk, Arpana Gupta, Berge Osnes, Elisabeth Kjelsvik Steinsvik, Arvid Lundervold, Gülen Arslan Lied, Astri J. Lundervold, Erica Persson Teige, Odd Helge Gilja, Judit Haász, Birgitte Berentsen, Jørgen Valeur, Bharath Halandur Nagaraja, Trygve Hausken, Jennifer S. Labus, Emeran A. Mayer |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Adolescent Disease Gut flora Bioinformatics Irritable Bowel Syndrome 03 medical and health sciences Diet Carbohydrate-Restricted Young Adult 0302 clinical medicine Cognition medicine Humans 030212 general & internal medicine Prospective Studies Prospective cohort study Irritable bowel syndrome Aged chemistry.chemical_classification biology business.industry Case-control study Brain General Medicine Middle Aged medicine.disease biology.organism_classification Gastrointestinal Microbiome chemistry 030220 oncology & carcinogenesis Case-Control Studies Fermentation Etiology Connectome Female business FODMAP |
| Zdroj: | p e21950 Medicine 99:e21950 |
| Popis: | Introduction Irritable bowel syndrome (IBS) is a common clinical label for medically unexplained gastrointestinal (GI) symptoms, recently described as a disturbance of the brain-gut-microbiota (BGM) axis. To gain a better understanding of the mechanisms underlying the poorly understood etiology of IBS, we have designed a multifaceted study that aim to stratify the complex interaction and dysfunction between the brain, the gut, and the microbiota in patients with IBS. Methods Deep phenotyping data from patients with IBS (n = 100) and healthy age- (between 18 and 65) and gender-matched controls (n = 40) will be collected between May 2019 and December 2021. Psychometric tests, questionnaires, human biological tissue/samples (blood, faeces, saliva, and GI biopsies from antrum, duodenum, and sigmoid colon), assessment of gastric accommodation and emptying using transabdominal ultrasound, vagal activity, and functional and structural magnetic resonance imaging (MRI) of the brain, are included in the investigation of each participant. A subgroup of 60 patients with IBS-D will be further included in a 12-week low FODMAP dietary intervention-study to determine short and long-term effects of diet on GI symptoms, microbiota composition and functions, molecular GI signatures, cognitive, emotional and social functions, and structural and functional brain signatures. Deep machine learning, prediction tools, and big data analyses will be used for multivariate analyses allowing disease stratification and diagnostic biomarker detection. Discussion To our knowledge, this is the first study to employ unsupervised machine learning techniques and incorporate systems-based interactions between the central and the peripheral components of the brain-gut-microbiota axis at the levels of the multiomics, microbiota profiles, and brain connectome of a cohort of 100 patients with IBS and matched controls; study long-term safety and efficacy of the low-FODMAP diet on changes in nutritional status, gut microbiota composition, and metabolites; and to investigate changes in the brain and gut connectome after 12 weeks strict low-FODMAP-diet in patients with IBS. However, there are also limitations to the study. As a restrictive diet, the low-FODMAP diet carries risks of nutritional inadequacy and may foster disordered eating patterns. Strict FODMAP restriction induces a potentially unfavourable gut microbiota, although the health effects are unknown. Trial registration number NCT04296552 (ClinicalTrials.gov). |
| Databáze: | OpenAIRE |
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