Ion-spray mass spectrometry for identification of the nonreducing terminal sugar of glycosaminoglycan
| Autor: | Masahiko Endo, Hideki Matsuya, Keiichi Takagaki, Wataru Nakamura, Mitsuo Majima, Hidekazu Munakata |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Molecular Sequence Data Carbohydrates Hyaluronoglucosaminidase Oligosaccharides Spectrometry Mass Secondary Ion Tandem mass spectrometry Mass spectrometry Biochemistry Mass Spectrometry Umbilical Cord Glycosaminoglycan chemistry.chemical_compound Fragmentation (mass spectrometry) Testis Hyaluronic acid Escherichia coli Animals Humans Hyaluronic Acid Glucuronidase Glycosaminoglycans chemistry.chemical_classification Chromatography Chemistry Glycosidic bond Glucuronic acid Acetylglucosamine Carbohydrate Sequence Cattle |
| Zdroj: | Glycobiology. 8:719-724 |
| ISSN: | 1460-2423 0959-6658 |
| DOI: | 10.1093/glycob/8.7.719 |
| Popis: | Various oligosaccharides from hyaluronic acid, which have glucuronic acid or N- acetylglucosamine at the nonreducing terminal, were prepared by digestion with a combination of testicular hyaluronidase and beta-glucuronidase. These oligo saccharides were analyzed by negative-mode ion-spray mass spectrometry (MS) with an atmospheric pressure ion source. Introduction of collisionally activated dissociation tandem mass spectrometry (CAD-MS/MS) produced ions derived from cleavage of the glycosidic bonds, allowing the structure to be analyzed. The CAD-MS/MS spectrum showed an intense and characteristic fragment ion at m/z 193 for oligosaccharides having glucuronic acid at the nonreducing terminal. On the other hand, this ion was not observed in the spectra of oligosaccharides having N- acetylglucosamine at the nonreducing terminal. Therefore, the fragmentation pattern revealed by CAD-MS/MS provides useful information for distinguishing glucuronic acid and N- acetylglucosamine at the nonreducing terminal of oligosaccharides derived from hyaluronic acid and other glycosaminoglycans. This ion-spray CAD-MS/MS technique was also applied successfully to the characterization of glycosaminoglycans reconstructed by glycotechnology. |
| Databáze: | OpenAIRE |
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