Dynamic Model for Biomass and Proteins Production by Three Bacillus Thuringiensis ssp Kurstaki Strains
| Autor: | Tatiana Segura Monroy, Julien Cescut, César Arturo Aceves-Lara, Jihane Saad, Mireille Kallassy, Souad Rouis, Nouha Abdelmalek, Nadia Bensaid, Joanna Abboud, Luc Fillaudeau |
|---|---|
| Přispěvatelé: | Toulouse Biotechnology Institute (TBI), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoires Medis, Centre de Biotechnologie de Sfax (CBS), Université Saint-Joseph de Beyrouth (USJ), Toulouse White Biotechnology (TWB), European Project: 734921 ,IPM-4-Citrus (2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
biopesticides
[SDV.BIO]Life Sciences [q-bio]/Biotechnology biology Process Chemistry and Technology Microorganism Chemical technology B. thuringiensis kurstaki kinetic parameters Biomass Bioengineering TP1-1185 biology.organism_classification dynamic model Spore Biopesticide Chemistry Bacillus thuringiensis Bioreactor Chemical Engineering (miscellaneous) Food science Akaike information criterion QD1-999 Total protein |
| Zdroj: | Processes Processes, 2021, 9 (12), pp.2147. ⟨10.3390/pr9122147⟩ Processes; Volume 9; Issue 12; Pages: 2147 Processes, Vol 9, Iss 2147, p 2147 (2021) |
| ISSN: | 2227-9717 |
| DOI: | 10.3390/pr9122147⟩ |
| Popis: | International audience; Bacillus thuringiensis is a microorganism used for the production of biopesticides worldwide. In the present paper, different kinetic models were analyzed to study and compare three different strains of Bt ssp kurstaki (LIP, BLB1, and HD1). Bioperformances (vegetative cell, spore, substrate, and protein) and successive culture phases (oxidative growth, limitation and sporulation, and protein release) were depicted with an overarching aim to estimate total protein productivity, yield, and titer. In the end, two models were calibrated using experimental dataset (11 batches culture in 3 L bioreactor with semisynthetic medium), subsequently validated, and statistically compared. Both models satisfactorily followed the dynamics of the experimental data. Finally, a dynamic model was selected following the Akaike information criterion (AIC). |
| Databáze: | OpenAIRE |
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