| Autor: |
Matthew B, Rogers, Dennis, Simon, Brian, Firek, Laurie, Silfies, Anthony, Fabio, Michael J, Bell, Andrew, Yeh, Justin, Azar, Richard, Cheek, Patrick M, Kochanek, Shyamal D, Peddada, Michael J, Morowitz |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Pediatr Crit Care Med |
| ISSN: |
1529-7535 |
| DOI: |
10.1097/pcc.0000000000002929 |
| Popis: |
OBJECTIVE: The microbiome may be affected by trauma and critical illness. Many studies of the microbiome in critical illness are restricted to a single body site or timepoint and confounded by pre-existing conditions. We report temporal and spatial alterations in the microbiome of previously healthy children with severe traumatic brain injury (TBI). DESIGN: We collected oral, rectal, and skin swabs within 72 hours of admission and then twice weekly until ICU discharge. Samples were analyzed by 16S rRNA gene amplicon sequencing. Children undergoing elective outpatient surgery served as controls. Alpha and beta diversity comparisons were performed with Phyloseq and differentially abundant taxa were predicted using ANCOM. SETTING: Five quaternary care pediatric intensive care units PATIENTS: Patients < 18 years with severe TBI requiring placement of an intracranial pressure (ICP) monitor INTERVENTIONS: None MEASUREMENTS AND MAIN RESULTS: 327 samples were analyzed from 23 children with severe TBI and 35 controls. The community composition of initial oral (F=3.2756, R(2)=0.0535, p=0.012) and rectal (F=3.0702, R(2)=0.0649, p=0.007) samples differed between TBI and control patients. Rectal samples were depleted of commensal bacteria from Ruminococcaceae, Bacteroidaceae, and Lachnospiraceae families and enriched in Staphylococcaceae after TBI (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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