Serotonin inputs to the dorsal BNST modulate anxiety in a 5-HT1A receptor dependent manner
| Autor: | Alvaro L. Garcia-Garcia, Mark S. Ansorge, Alex Dranovsky, Nesha S. Burghardt, Sarah E. Canetta, J M Stujenske, E D Leonardo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Serotonin medicine.drug_class Optogenetics Anxiety Anxiolytic Article 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Extended amygdala medicine Animals Molecular Biology 5-HT receptor Neurons Anxiety Disorders Mice Inbred C57BL Psychiatry and Mental health Stria terminalis 030104 developmental biology Anxiogenic Receptor Serotonin 5-HT1A Raphe Nuclei Septal Nuclei Psychology Raphe nuclei Neuroscience 030217 neurology & neurosurgery Serotonergic Neurons |
| Zdroj: | Molecular psychiatry |
| ISSN: | 1476-5578 1359-4184 |
| Popis: | Serotonin (5-HT) neurons project from the raphe nuclei throughout the brain where they act to maintain homeostasis. Here, we study 5-HT inputs into the bed nucleus of the stria terminalis (BNST), a major subdivision of the extended amygdala that has been proposed to regulate responses to anxiogenic environments in humans and rodents. While the dorsal part of the BNST (dBNST) receives dense 5-HT innervation, whether and how 5-HT in the dBNST normally modulates anxiety remains unclear. Using optogenetics, we demonstrate that activation of 5-HT terminals in the dBNST reduces anxiety in a highly anxiogenic environment. Further analysis revealed that optogenetic inhibition of 5-HT inputs into the dBNST increases anxiety in a less anxiogenic environment. We found that 5-HT predominantly hyperpolarizes dBNST neurons, reducing their activity in a manner that can be blocked by a 5-HT1A antagonist. Finally, we demonstrate that activation of 5-HT1A receptors in the dBNST is necessary for the anxiolytic effect observed following optogenetic stimulation of 5-HT inputs into the dBNST. These data reveal that 5-HT release in the dBNST modulates anxiety-like behavior via 5-HT1A receptors under naturalistic conditions. |
| Databáze: | OpenAIRE |
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