Determination of the structural role of the N-terminal domain of human extracellular superoxide dismutase by use of protein fusions
| Autor: | Eleonore Skärfstad, Bengt-Harald Jonsson, Lena A. E. Tibell |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Protein Conformation
Recombinant Fusion Proteins Molecular Sequence Data Biophysics Biochemistry Chromatography Affinity Protein Structure Secondary Domain (software engineering) Superoxide dismutase chemistry.chemical_compound Affinity chromatography Structural Biology Carbonic anhydrase Escherichia coli Humans Molecule Amino Acid Sequence Molecular Biology Conserved Sequence Carbonic Anhydrases DNA Primers Base Sequence biology Superoxide Dismutase Circular Dichroism Fusion protein Protein Structure Tertiary Molecular Weight Monomer chemistry Chromatography Gel Mutagenesis Site-Directed biology.protein Electrophoresis Polyacrylamide Gel Heterologous expression |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1292:47-52 |
| ISSN: | 0167-4838 |
| DOI: | 10.1016/0167-4838(95)00189-1 |
| Popis: | The N-terminal domain, containing the 49 N-terminal amino-acid residues, of human extracellular superoxide dismutase (hEC-SOD) has been studied after construction of fusion proteins comprised of the defined domain and human carbonic anhydrase 11 (HCAII). The specific advantage of this technique is that it allows characterization of properties that are intrinsic to the N-terminal domain of hEC-SOD, i.e., the results are not obscured by properties pertaining to the rest of the hEC-SOD molecule. Moreover, the fusion to HCAII allows a rapid and gentle one-step purification by affinity chromatography. When the N-terminal domain was fused to the N-terminal of HCAII (=FusNN) a well defined structure was formed and the resulting protein was tetrameric. When the same hEC-SOD-derived domain was fused to the C-terminal of HCAII (=FusNC), no defined structure of the fused domain could be observed, and the resulting protein was monomeric. It was concluded that a ‘free’ N-terminus is required for formation of the proper structure of the N-terminal domain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect