Using ambient ozone for assignment of double bond position in unsaturated lipids
| Autor: | Jessica R Hughes, Todd W. Mitchell, Stephen J. Blanksby, Marc in het Panhuis, Shane R. Ellis |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Desorption electrospray ionization Spectrometry Mass Electrospray Ionization Chromatography Ozonolysis Double bond Electrospray ionization Lipidome Mass spectrometry Biochemistry Lipids Analytical Chemistry Ozone chemistry Isomerism Lens Crystalline Electrochemistry Mass spectrum Environmental Chemistry Humans Lactosylceramides Chromatography Thin Layer Spectroscopy |
| Zdroj: | The Analyst. 137(5) |
| ISSN: | 1364-5528 |
| Popis: | Unsaturated lipids deposited onto a range of materials are observed to react with the low concentrations of ozone present in normal laboratory air. Parent lipids and ozonolysis cleavage products are both detected directly from surfaces by desorption electrospray ionisation mass spectrometry (DESI-MS) with the resulting mass spectra providing clear evidence of the double bond position within these molecules. This serendipitous process has been coupled with thin-layer chromatography (TLC) to provide a simple but powerful approach for the detailed structural elucidation of lipids present in complex biological extracts. Lipid extracts from human lens were deposited onto normal phase TLC plates and then developed to separate components according to lipid class. Exposure of the developed plates to laboratory air for ca. 1 h prior to DESI-MS analysis gave rise to ozonolysis products allowing for the unambiguous identification of double bond positions in even low abundant, unsaturated lipids. In particular, the co-localization of intact unsaturated lactosylceramides (LacCer) with products from their oxidative cleavage provide the first evidence for the presence of three isomeric LacCer (d18:0/24:1) species in the ocular lens lipidome, i.e., variants with double bonds at the n-9, n-7 and n-5 positions. |
| Databáze: | OpenAIRE |
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