GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage
| Autor: | Michael G. Hahn, Beth Richardson, Yingzhen Kong, James Schafhauser, Debra Mohnen, Louise Wicker, Gongke Zhou, Ashraf Abdeen, Melani A. Atmodjo, Tamara L. Western, Jiyoung Jung |
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| Rok vydání: | 2013 |
| Předmět: |
Physiology
Green Fluorescent Proteins Mutant Arabidopsis Golgi Apparatus Plant Science Biology Endoplasmic Reticulum Rhamnose Plant Mucilage Biochemistry and Metabolism Gene Expression Regulation Plant Genetics Arabidopsis thaliana In Situ Hybridization Oligonucleotide Array Sequence Analysis Microscopy Confocal Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Hexuronic Acids Genetic Complementation Test Wild type food and beverages Plants Genetically Modified biology.organism_classification Complementation Transformation (genetics) Biochemistry Mucilage Mutation Seeds Microscopy Electron Scanning Pectins Silique Transcriptome |
| Zdroj: | Plant Physiology. 163:1203-1217 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.113.227041 |
| Popis: | The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I. |
| Databáze: | OpenAIRE |
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