Improved Culture Method for the Isolation ofHistoplasma capsulatumandBlastomyces dermatitidisfrom Contaminated Specimens
| Autor: | Norman L. Goodman, Coy D. Smith |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
food.ingredient
Histoplasma Mycology Fungus Microbiology Agar plate Dogs food Hydroxides medicine Animals Humans Agar biology Blastomyces dermatitidis Sputum General Medicine Contamination Isolation (microbiology) biology.organism_classification Culture Media Quaternary Ammonium Compounds Blastomyces medicine.symptom Bacteria |
| Zdroj: | American Journal of Clinical Pathology. 63:276-280 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/63.2.276 |
| Popis: | Studies were performed to evaluate a method for selective isolation of Histoplasma capsulatum and Blastomyces dermatitidis from contaminated specimens. Ammonium hydroxide placed on an agar medium surface was found to inhibit the growth of many bacteria, yeasts, and saphrophytic fungi normally found in specimens such as animal tissues and sputum. In one study involving the culture of B. dermatitidis from canine tissues, 24% more isolations were obtained on a medium using NH4OH compared with a similar medium. Increases in the isolation of H. capusulatum from sputum specimens were also obtained, ranging from 20 to 32% compared with four other media without NH4OH. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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