Ultrastructural, immunocytochemical, and cytogenetic characterization of a human epithelioid sarcoma cell line (RM-HS1)
| Autor: | Courtenay Vd, Robertson D, Reeves Br, S Smith, Fisher C |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_specialty Pathology Epithelioid sarcoma Aneuploidy Vimentin Biology Cell Line Foot Diseases Antigens Neoplasm Keratin medicine Humans Intermediate filament chemistry.chemical_classification Chromosome Aberrations Mucin-1 Cytogenetics Membrane Proteins Karyotype Sarcoma medicine.disease Neoplasm Proteins ErbB Receptors Oncology chemistry Cell culture biology.protein Keratins |
| Zdroj: | Journal of the National Cancer Institute. 78(1) |
| ISSN: | 0027-8874 |
| Popis: | A cell line (RM-HS1) derived from a human epithelioid sarcoma was established in tissue culture. Ultrastructurally, the cells show features of those found within the primary tumor. A mixed mesenchymal-epithelial phenotype, defined by reactivity with antibodies to epithelial membrane antigen and to vimentin and keratin intermediate filaments, was found in the tumor, and a similar phenotype persisted in the cultured cells. Cytogenetic analysis revealed a mode of 66 chromosomes. With the use of a variety of banding techniques together with in situ hybridization of a 3H-labeled molecular probe for 18s and 28s ribosomal RNA genes (pX1r101), the karyotypes were shown to contain extensive numerical and structural rearrangements, with up to 24 marker chromosomes. |
| Databáze: | OpenAIRE |
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