Establishing an efficient explant superficial sterilization protocol for in vitro micropropagation of bear’s garlic (Allium ursinum L.)
| Autor: | Anna Figas, Anna J. Keutgen, Magdalena Tomaszewska-Sowa, Norbert Keutgen |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
sterylizacja
Traditional medicine in vitro cultures kultury in vitro Pharmacology toxicology food and beverages UV–C irradiation Sterilization (microbiology) Biology lcsh:Plant culture food.food sterilization procedures Allium ursinum L bear’s garlic food Micropropagation Allium ursinum Botany lcsh:SB1-1110 czosnek niedźwiedzi promieniowanie UV-C Explant culture |
| Zdroj: | Herba Polonica, Vol 61, Iss 4, Pp 66-77 (2015) |
| ISSN: | 2449-8343 |
| Popis: | Summary Introduction: Allium ursinum L. has a commercial value due to its high contents of bio-active compounds and mild, garlic-like taste. In vitro culture played an important role in obtaining Allium species with the desired characteristics and in the production of healthy reproductive material. Objective: Developing an effective method of sterilization bear’s garlic bulbs. Methods: To obtain sterile shoots of garlic several methods of sterilization involving such factors as ACE, H2O2, HgCl2, and UV-C were tested. Results: In order to obtain sterile shoots of bear’s garlic, several sterilization procedures were tested. The best procedure was based on a two-step disinfection, where the whole onions were treated with ethanol and H2O2 for 20 min. Thereafter, the isolated apical buds were sterilized in ACE for 10 min, rinsed in double-distilled water and transferred onto MS medium for growing. Up to 95% of the inoculated explants formed shoots, which were sub-cultured on MS with 4 mg dm−3 BAP in order to enable further propagation. Conclusion: After optimization and stabilization, this procedure may become the basic concept of a proper and reliable propagation method of bear’s garlic on commercial scale. |
| Databáze: | OpenAIRE |
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