Roles of miR-210 in the pathogenesis of pre-eclampsia
| Autor: | Ji-Yun Li, Yan-Min Cao, Zhi Hou, Gui-Mei Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
MAPK/ERK pathway
pre-eclampsia migration miR-210 Andrology 03 medical and health sciences 0302 clinical medicine medicine MTT assay 030212 general & internal medicine Protein kinase B biology Cell growth business.industry General Medicine Hypoxia (medical) invasion microrna210 Blot Basic Research biology.protein Medicine Phosphorylation medicine.symptom PTPN2 business Platelet-derived growth factor receptor |
| Zdroj: | Archives of Medical Science : AMS Archives of Medical Science, Vol 15, Iss 1, Pp 183-190 (2018) |
| ISSN: | 1896-9151 1734-1922 |
| Popis: | Introduction This study aimed to explore the bio-function of miR-210 in the pathogenesis of pre-eclampsia and provide new insights into the diagnosis and treatment of pre-eclampsia. Material and methods A JAR cell line cultured in standard or hypoxic conditions was used in this study. Expression levels of miR-210 and PTPN2 were determined using real-time polymerase chain reaction (RT-PCR). Protein and phosphorylation levels were assessed using western blotting. Proliferation of JAR cells was evaluated using MTT assay. Migration and invasion were measured using transwell assay. Results Expression of miR-210 increased significantly in a time-dependent manner after hypoxia treatment within 36 h (p < 0.05). miR-210 inhibitor significantly decreased the cell proliferation, migration, and invasion (p < 0.05), while miR-210 mimic reversed these findings (p < 0.05). Hypoxia significantly suppressed the expression of PTPN2; however, this elevation was abolished by miR-210 inhibitor (p < 0.05). Inhibition of PTPN2 or hypoxia significantly increased the proliferation, migration, and invasion of JAR cells, while miR-210 inhibitor significantly reversed these changes (p < 0.05). The phosphorylation levels of PDGFR, Akt, and Erk were markedly upregulated by hypoxia or si-PTPN2, but this effect was abolished by miR-210 inhibitor (p < 0.05). Conclusions miR-210 can promote proliferation, migration, and invasion via downregulating PTPN2 in the PDGFR-Akt pathway |
| Databáze: | OpenAIRE |
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