Cell surface labeling and mass spectrometry reveal diversity of cell surface markers and signaling molecules expressed in undifferentiated mouse embryonic stem cells
| Autor: | Masato Taoka, Toshiaki Isobe, Kazuto Nunomura, Tomonori Izumi, Kohji Nagano, Yoshio Yamauchi, Nobuhiro Takahashi, Sumio Sugano, Chiharu Itagaki, Nobuko Okamura |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Cell signaling
Cellular differentiation Integrin Biology Cell Fractionation Biochemistry Mass Spectrometry Analytical Chemistry Cell Line Automation Mice Animals Biotinylation Amino Acid Sequence Molecular Biology DNA Primers Cluster of differentiation Base Sequence Cell adhesion molecule Stem Cells Cell Membrane Wnt signaling pathway Membrane Proteins Embryo Mammalian Embryonic stem cell Peptide Fragments Cell biology biology.protein Signal transduction Biomarkers Signal Transduction |
| Zdroj: | Molecularcellular proteomics : MCP. 4(12) |
| ISSN: | 1535-9476 |
| Popis: | Although interactions between cell surface proteins and extracellular ligands are key to initiating embryonic stem cell differentiation to specific cell lineages, the plasma membrane protein components of these cells are largely unknown. We describe here a group of proteins expressed on the surface of the undifferentiated mouse embryonic stem cell line D3. These proteins were identified using a combination of cell surface labeling with biotin, subcellular fractionation of plasma membranes, and mass spectrometry-based protein identification technology. From 965 unique peptides carrying biotin labels, we assigned 324 proteins including 235 proteins that have putative signal sequences and/or transmembrane segments. Receptors, transporters, and cell adhesion molecules were the major classes of proteins identified. Besides known cell surface markers of embryonic stem cells, such as alkaline phosphatase, the analysis identified 59 clusters of differentiation-related molecules and more than 80 components of multiple cell signaling pathways that are characteristic of a number of different cell lineages. We identified receptors for leukemia-inhibitory factor, interleukin 6, and bone morphogenetic protein, which play critical roles in the maintenance of undifferentiated mouse embryonic stem cells. We also identified receptors for growth factors/cytokines, such as fibroblast growth factor, platelet-derived growth factor, ephrin, Hedgehog, and Wnt, which transduce signals for cell differentiation and embryonic development. Finally we identified a variety of integrins, cell adhesion molecules, and matrix metalloproteases. These results suggest that D3 cells express diverse cell surface proteins that function to maintain pluripotency, enabling cells to respond to various external signals that initiate differentiation into a variety of cell types. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|