The use of an enzyme-linked immunosorbent assay for estimation of the immune status of chickens artificially immunized against coccidiosis
| Autor: | Y. Kudo, Toshio Ishii, H. Onaga, T. Motohashi, M. Togo |
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| Rok vydání: | 1989 |
| Předmět: |
Veterinary medicine
animal structures animal diseases Antibodies Protozoan Antigens Protozoan Enzyme-Linked Immunosorbent Assay Eimeria Excretion Coccidia Antigen Immunity parasitic diseases medicine Animals Poultry Diseases chemistry.chemical_classification Immune status General Veterinary biology Coccidiosis General Medicine biology.organism_classification medicine.disease Virology Enzyme chemistry embryonic structures Immunization Parasitology Chickens |
| Zdroj: | Veterinary Parasitology. 33:199-205 |
| ISSN: | 0304-4017 |
| Popis: | Nine thousand commercial breeder chicks (Chankee) reared in a floor pen were exposed to restricted numbers of Eimeria tenella and E. necatrix oocysts to confer immunity. Antibody induction in these chicks was examined by the enzyme-linked immunosorbent assay (ELISA) with antigen prepared from E. tenella oocysts. The oocyst excretion pattern demonstrated recycled infections which continued in these chicks for greater than or equal to 22 days after exposure. Antibody levels in their sera, as determined by the mean absorbence values in ELISA, increased gradually up to 38 days post-inoculation. Mean absorbence values of sera from control chicks remained at a low level. When infected and control chicks were challenged with the two species of coccidia, the test chicks were protected against both species. The antibody level did not change for 8 days in the challenge groups, while in the control chicks, absorbence in ELISA rose significantly and the mean absorbence value was higher than that in immunized chicks. Some factors which influence the results of ELISA are considered and the applicability of this method to measuring immunity against coccidiosis in chickens is discussed. |
| Databáze: | OpenAIRE |
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