Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver of Pelophylax esculentus complex frogs
| Autor: | Slavica Borković-Mitić, Branka R. Gavrilović, Marko D. Prokić, Slađan Z. Pavlović, Imre Krizmanić, Zorica S. Saičić, Svetlana G. Despotović, Tijana B. Radovanović, Jelena P. Gavrić |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pelophylax Pelophylax ridibundus Glutathione reductase Pelophylax esculentus complex 010501 environmental sciences Biology medicine.disease_cause 01 natural sciences Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound biology.animal Botany medicine lcsh:QH301-705.5 0105 earth and related environmental sciences chemistry.chemical_classification Agricultural and Biological Sciences(all) Glutathione peroxidase Glutathione biology.organism_classification Molecular biology 030104 developmental biology chemistry Liver lcsh:Biology (General) Catalase Oxidative stress biology.protein Acetylcholinesterase Frogs Original Article General Agricultural and Biological Sciences Antioxidative defense system |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 24, Iss 1, Pp 51-58 (2017) Saudi Journal of Biological Sciences |
| Popis: | Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (–SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to the Pelophylax esculentus “complex” (parental species Pelophylax ridibundus, Pelophylax lessonae, and their hybrid Pelophylax kl. esculentus) from the wetland, Obedska bara in Serbia. The condition factor (CF) and hepato somatic index (HSI) were also calculated. All three species were caught at same locality and were exposed to the same environmental conditions. Liver SOD activity was lower in P. ridibundus than in P. kl. esculentus and P. lessonae; higher activities of CAT, GR and GST were observed in P. kl. esculentus frogs as compared to their parental species. The activity of GSH-Px was significantly lower in P. kl. esculentus. The activity of AChE was increased in P. lessonae as compared to P. kl. esculentus and P. ridibundus. Similar concentrations of GSH and SH groups were observed in all investigated species. P. kl. esculentus had a higher CF, while the HSI was lower when compared to the parental species. Our findings suggest that the parental species (P. ridibundus and P. lessonae) possess more similar antioxidative responses to environmental conditions than the hybrid species P. kl. esculentus. The obtained results improve our understanding of the biology and physiology of these three closely related species. |
| Databáze: | OpenAIRE |
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