Population Genetics of Verticillium dahliae in Iran Based on Microsatellite and Single Nucleotide Polymorphism Markers
| Autor: | B. Gillian Turgeon, Laura S. Bautista-Jalon, Michael G. Milgroom, Vahideh Rafiei, Ziaeddin Banihashemi, María del Mar Jiménez-Gasco |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Lineage (genetic) Population Population genetics Plant Science Iran Verticillium Biology Polymorphism Single Nucleotide 01 natural sciences Coalescent theory 03 medical and health sciences Verticillium dahliae DNA Fungal education Genetics education.field_of_study Haplotype Fungal genetics food and beverages biology.organism_classification 030104 developmental biology Verticillium wilt Agronomy and Crop Science Microsatellite Repeats 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 108:780-788 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto-11-17-0360-r |
| Popis: | Verticillium dahliae is a plant pathogenic fungus that reproduces asexually and its population structure is highly clonal. In the present study, 78 V. dahliae isolates from Iran were genotyped for mating type, single nucleotide polymorphisms (SNPs), and microsatellites to assign them to clonal lineages and to determine population genetic structure in Iran. The mating type of all isolates was MAT1-2. Based on neighbor-joining analysis and minimum spanning networks constructed from SNPs and microsatellite genotypes, respectively, all but four isolates were assigned to lineage 2B824; four isolates were assigned to lineage 4B. The inferred coalescent genealogy of isolates in lineage 2B824 showed a clear divergence into two clades that corresponded to geographic origin and host. Haplotypes of cotton and pistachio isolates sampled from central Iran were in one clade, and those of isolates from Prunus spp. sampled from northwestern Iran were in the other. The strong divergence in haplotypes between the two clades suggests that there were at least two separate introductions of lineage 2B824 to different parts of Iran. Given the history of cotton and pistachio cultivation and Verticillium wilt in Iran, these results are consistent with the hypothesis that cotton was historically a likely source inoculum causing Verticillium wilt in pistachio. |
| Databáze: | OpenAIRE |
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