Validation of the BD FACSPresto system for the measurement of CD4 T-lymphocytes and hemoglobin concentration in HIV-negative and HIV-positive subjects

Autor:	Hao Wu, Tong Zhang, Huiming Sheng, Zhaoqin Zhu, Haicong Li, Shijun Yu, Hanxiao Sun, Daihong Chen, Wei Xia, Xiaofan Lu, Meiyu Tan
Rok vydání:	2020
Předmět:	0301 basic medicine Adult Male medicine.medical_specialty Point-of-Care Systems Human immunodeficiency virus (HIV) lcsh:Medicine HIV Infections medicine.disease_cause Gastroenterology Article 03 medical and health sciences Deming regression Hemoglobins 0302 clinical medicine Hematology analyzer Pregnancy Internal medicine HIV Seropositivity medicine Humans 030212 general & internal medicine Prospective Studies lcsh:Science Prospective cohort study Reference standards Multidisciplinary business.industry lcsh:R Biological techniques Venous blood Flow Cytometry CD4 Lymphocyte Count 030104 developmental biology Method comparison lcsh:Q Female Hemoglobin business Biotechnology
Zdroj:	Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
ISSN:	2045-2322
Popis:	This study aimed to compare the performance of the BD FACSPresto system with the conventional standard-of-care technologies for the measurement of absolute CD4 count (AbsCD4), CD4 percentage (CD4%) and total hemoglobin concentration (Hb) in capillary and venous blood samples of HIV-negative and HIV-positive subjects. A total of 1304 participants were included in this prospective cohort study. Both venous and capillary blood samples were analyzed using the BD FACSPresto system and the results were compared against the BD FACSCalibur for enumerating AbsCD4 and CD4% and Sysmex XT-4000i hematology analyzer for determining Hb levels. Method comparison studies were performed using Deming regression and Bland–Altman plots. The Deming regression analyses comparing the accuracy of the BD FACSPresto system with the reference standard technologies demonstrated a significant linear correlation between the AbsCD4, CD4%, and Hb values generated by the two platforms. The 95% CI of the slopes for AbsCD4, CD4%, and Hb levels were 0.94–0.99, 0.99–1.01 and 0.86–0.93, respectively (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::854501cb5649f81442b943aac8408527 https://pubmed.ncbi.nlm.nih.gov/33177659 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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