Unraveling peroxynitrite formation in biological systems
| Autor: | María Noel Alvarez, Rafael Radi, Alfonso Cayota, Gonzalo Peluffo, Mercedes Naviliat |
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| Rok vydání: | 2001 |
| Předmět: |
Free Radicals
Cytochrome c Group Hydroxylation Biochemistry Nitric oxide Oxidative damage chemistry.chemical_compound Phenols Physiology (medical) Animals Humans Tyrosine Metabolism Fluorescent Dyes Nitrates Chemistry Superoxide Electron Spin Resonance Spectroscopy Carbon Dioxide Oxidants Multiple target Immunohistochemistry Oxidative Stress 3-nitrotyrosine Luminescent Measurements Biophysics Tyrosine Indicators and Reagents Peroxynitrite |
| Zdroj: | Free Radical Biology and Medicine. 30:463-488 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(00)00373-7 |
| Popis: | Peroxynitrite promotes oxidative damage and is implicated in the pathophysiology of various diseases that involve accelerated rates of nitric oxide and superoxide formation. The unambiguous detection of peroxynitrite in biological systems is, however, difficult due to the combination of a short biological half-life, limited diffusion, multiple target molecule reactions, and participation of alternative oxidation/nitration pathways. In this review, we provide the conceptual framework and a comprehensive analysis of the current experimental strategies that can serve to unequivocally define the existence and quantitation of peroxynitrite in biological systems of different levels of organization and complexity. |
| Databáze: | OpenAIRE |
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