Vitamin E modulates apoptosis and c-jun N-terminal kinase activation in ovarian torsion–detorsion injury
| Autor: | Gulnur Kizilay, İmran Kurt Ömürlü, Meryem Akpolat, Yeter Topcu-Tarladacalisir, Mustafa Inan, Yesim Hulya Uz, Aysegul Cerkezkayabekir, Melike Sapmaz-Metin |
|---|---|
| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi |
| Rok vydání: | 2013 |
| Předmět: |
JNK MAPK
Torsion Abnormality medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Apoptosis Ovary Antioxidants Pathology and Forensic Medicine Lipid peroxidation chemistry.chemical_compound Internal medicine Follicular phase In Situ Nick-End Labeling medicine Animals Vitamin E Ovarian Diseases Rats Wistar Molecular Biology Torsion-detorsion biology business.industry JNK Mitogen-Activated Protein Kinases Ovarian torsion medicine.disease Malondialdehyde Immunohistochemistry Rats Enzyme Activation Disease Models Animal medicine.anatomical_structure Endocrinology chemistry Reperfusion Injury Myeloperoxidase biology.protein Rat Female Lipid Peroxidation business |
| Zdroj: | Experimental and Molecular Pathology. 95:213-219 |
| ISSN: | 0014-4800 |
| DOI: | 10.1016/j.yexmp.2013.07.007 |
| Popis: | The aim of this study was to evaluate the role of vitamin E in follicular degeneration and to assess histopathological and biochemical changes following ischemia-reperfusion (IR) injury in rat ovaries. Twenty-eight Wistar albino rats were randomly divided into four groups: sham, 4. h torsion, 24. h detorsion, and a vitamin E group. Thirty minutes before detorsion, a single dose of 200. mg/kg vitamin E was administered intraperitoneally. The ovarian histology score was determined, serum levels of malondialdehyde (MDA) and myeloperoxidase (MPO) were measured. The apoptosis of granulosa cells and the phospho-c-jun N-terminal kinase (p-JNK) and phospho-p38 (p-p38) immunoreactivities of these cells were determined. MDA and MPO levels were significantly increased in the torsion and detorsion groups. Hemorrhage, edema, and congestion were also apparent in these groups. In addition, the apoptotic index and the immunoreactivity of p-JNK were highest in the detorsion group, which also showed marked follicular degeneration. However, p-p38 activity was not affected by torsion-detorsion (TD) induction. Vitamin E ameliorated TD-induced histological alterations. It also decreased serum levels of MDA and MPO, reduced the activity of p-JNK in the ovaries, and reduced numbers of apoptotic follicular cells. In conclusion, these data indicate that vitamin E attenuated ovarian follicular degeneration by inhibiting the immunoreactivity of p-JNK and reducing the apoptosis of granulosa cells. © 2013 Elsevier Inc. 2011-121 The authors acknowledge Trakya University Research Center, Edirne, Turkey for the financial support (project no: 2011-121 ). |
| Databáze: | OpenAIRE |
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