Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts
| Autor: | Jun Watanabe, Jun Takebayashi, Manabu Wagaki, Yuko Takano-Ishikawa, Akemi Yasui, Tomoyuki Oki, Hiroshi Yada |
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| Rok vydání: | 2016 |
| Předmět: |
Validation study
Antioxidant Cinnamomum zeylanicum Oxygen radical absorbance capacity Free Radicals medicine.medical_treatment alpha-Tocopherol Standard solution Ginger Antioxidants Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology medicine Chromans Reproducibility Chromatography Phenylpropionates Chemistry Lipophilic antioxidant Reproducibility of Results Oryza 04 agricultural and veterinary sciences 040401 food science Oxygen Solutions Antioxidant capacity Spectrometry Fluorescence Food Fluorescein Trolox Powders Reactive Oxygen Species Food Analysis |
| Zdroj: | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 32(2) |
| ISSN: | 1348-2246 |
| Popis: | A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein, radical generator, and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSD(int)) ranged from 7.0 to 16.7%, the reproducibility relative standard deviations (RSD(R)) ranged from 14.8 to 19.4%, and the HorRat values ranged from 1.35 to 1.78. |
| Databáze: | OpenAIRE |
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