Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides
| Autor: | Claude Durrieu, Christophe Védrine, Jean-Claude Leclerc, Canh Tran-Minh |
|---|---|
| Přispěvatelé: | Centre Sciences des Processus Industriels et Naturels (SPIN-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Département Poudres et Matériaux Multi-Composants (P2MC-ENSMSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-SPIN, Equipe d'Ecophysiologie (EE-FSSE), Faculté des Sciences de Saint-Etienne, Laboratoire des Sciences de l'Environnement (LSE-ENTPE), École Nationale des Travaux Publics de l'État (ENTPE)-Université de Lyon-Ministère de l'Ecologie, du Développement Durable, des Transports et du Logement, Université Jean Monnet, Ecole Nationale des Travaux Publics de l'Eta |
| Rok vydání: | 2003 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Algae Chlorella vulgaris Biomedical Engineering Biophysics Simazine Biosensing Techniques Chlorella Ecotoxicology Sensitivity and Specificity Fluorescence chemistry.chemical_compound Fluorometer Botany Electrochemistry Fiber Optic Technology [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Atrazine [INFO.INFO-BT]Computer Science [cs]/Biotechnology Chlorophyll fluorescence Cells Cultured Detection limit Chromatography Dose-Response Relationship Drug biology Herbicides Temperature Reproducibility of Results Equipment Design General Medicine Cells Immobilized Flow Cytometry biology.organism_classification chemistry Herbicide Biosensor Water Pollutants Chemical Environmental Monitoring Biotechnology |
| Zdroj: | Biosensors and Bioelectronics Biosensors and Bioelectronics, Elsevier, 2003, 18 (4), pp.547-463. ⟨10.1016/S0956-5663(02)00157-4⟩ |
| ISSN: | 0956-5663 |
| Popis: | International audience; An optical biosensor was designed for determination of herbicides as aquatic contaminants. Detection was obtained with immobilised Chlorella vulgaris microalgae entrapped on a quartz microfibre filter and placed in a five-membrane-home-made-flow cell. The algal chlorophyll fluorescence modified by the presence of herbicides was collected at the tip of an optical fibre bundle and sent to a fluorimeter. A continuous culture was set up to produce algal cells in reproducible conditions for measurement optimisation. Effects of flow rate, algal density, temperature, and pH on the biosensor response to atrazine were studied. Reversibility and detection limits were determined for DNOC and atrazine, simazine, isoproturon, diuron. Detection of photosystem II (PSII) herbicides was achieved at sub-ppb concentration level. |
| Databáze: | OpenAIRE |
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