Differential isotope labeling of 38 dietary polyphenols and their quantification in urine by liquid chromatography electrospray ionization tandem mass spectrometry
| Autor: | Augustin Scalbert, Cécile Cren-Olivé, David Achaintre, Sabina Rinaldi, Audrey Buleté, Liang Li |
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| Přispěvatelé: | Biomarkers Group, International Agency for Cancer Research (IACR), TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Dept Chem, University of Alberta, This work was supported by the Institut National du Cancer, Paris (INCa Grant #2011-105) and the Wereld Kanker Onderzoek Fonds (WCRF NL Grant #2012/604). |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Spectrometry Mass Electrospray Ionization SAMPLES Electrospray ionization BIOMARKERS Urine BEVERAGES Tandem mass spectrometry Mass spectrometry 01 natural sciences METABOLITES PHENOL-EXPLORER DATABASE Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry [CHIM.ANAL]Chemical Sciences/Analytical chemistry SYSTEMATIC ANALYSIS Carbon Radioisotopes Derivatization Detection limit Dansyl Compounds 030109 nutrition & dietetics Chromatography Chemistry 010401 analytical chemistry Dansyl chloride Polyphenols food and beverages FLAVONOIDS HUMANS 0104 chemical sciences 3. Good health Polyphenol RICH FOODS Indicators and Reagents UHPLC-ESI-MS/MS Chromatography Liquid |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2016, 88 (5), pp.2637-2644. ⟨10.1021/acs.analchem.5b03609⟩ |
| ISSN: | 0003-2700 1520-6882 |
| DOI: | 10.1021/acs.analchem.5b03609⟩ |
| Popis: | International audience; A large number of polyphenols are consumed with the diet and may contribute to the prevention of chronic diseases such as cardiovascular diseases, diabetes, cancers, and neurodegenerative diseases. More comprehensive methods are needed to measure exposure to this complex family of bioactive plant compounds in epidemiological studies. We report here a novel method enabling the simultaneous measurement in urine of 38 polyphenols representative of the main classes and subclasses found in the diet. This method is based on differential C-12-/C-13-isotope labeling of polyphenols through derivatization with isotopic dansyl chloride reagents and on the analysis of the labeled polyphenols by tandem mass spectrometry. This derivatization approach overcomes the need for costly labeled standards. Different conditions for enzyme hydrolysis of polyphenol glucuronides and sulfate esters, extraction, and dansylation of unconjugated aglycones were tested and optimized. Limits of quantification varied from 0.01 to 1.1 mu M depending on polyphenols. Intrabatch coefficients of variation varied between 3.9% and 9.6%. Interbatch variations were lower than 15% for 31 compounds and lower than 29% for 6 additional polyphenols out of the 38 tested. Thirty seven polyphenols were validated and then analyzed in 475, 24 h urine samples from the European Prospective Investigation on Cancer and Nutrition (EPIC) study. Thirty four polyphenols could be detected and successfully estimated and showed large interindividual variations of concentrations (2-3 orders of magnitude depending on the compound), with median concentrations spanning from 0.01 to over 1000 mu M for all 34 compounds. |
| Databáze: | OpenAIRE |
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