Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy
| Autor: | Takashi Tsuji, Tatsuo Sakai, Hiroyuki Matsumoto, Yuya Hidoh, Tomomi Nakamura, Tomoko Obara, Rebecca Powell, Shuji Terai, Yoshio Kodera, Koichiro Ichimura, Jian Xing Ma, Yusuke Kawashima, Isao Sakaida |
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| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
medicine.medical_specialty Oryzias Kidney Glomerulus Biophysics Adipose tissue Muscle Proteins Diet High-Fat Biochemistry Article Glomerulonephritis Glomerulopathy Internal medicine medicine Animals Humans Obesity Molecular Biology Zebrafish Metabolic Syndrome biology Myosin Heavy Chains fungi Microfilament Proteins food and beverages Cell Biology Organ Size biology.organism_classification medicine.disease Disease Models Animal Endocrinology Mesangium Steatohepatitis Metabolic syndrome |
| Zdroj: | Biochemical and biophysical research communications. 431(4) |
| ISSN: | 1090-2104 |
| Popis: | Obesity, an ongoing significant public health problem, is a part of complex disease characterized as metabolic syndrome. Medaka and zebrafish are useful aquatic experimental animals widely used in the field of toxicology and environmental health sciences and as a human disease models. In medaka, simple feeding of a high fat diet (HFD) can induce body weight gain, excessive accumulation of visceral adipose tissue, hyperglycemia, hyperlipidemia, and steatohepatitis, which mimics human metabolic syndrome. In the present study, to explore the possibility that the adult medaka fed with HFD (HFD-medaka) can be used as an animal model for human metabolic syndrome-associated glomerular disease, including obesity-related glomerulopathy (ORG), we analyzed structural alterations and protein expression in the mesonephric kidney of HFD-medaka. We found that the histopathology was consistent with glomerulomegaly accompanied by the dilation of glomerular capillaries and proliferative expansion of the mesangium, a condition partially comparable to human ORG. Moreover, expressions of several kinds of kidney disease-related proteins (such as MYH9, SM22α) were significantly elevated. Thus, the HFD-medaka has a high potential as an animal model useful for exploring the mechanism underling human ORG. |
| Databáze: | OpenAIRE |
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