Protective effect and induction of DNA repair by Myrciaria cauliflora seed extract and pedunculagin on cyclophosphamide-induced genotoxicity
| Autor: | C R Vale, Luciane Dias Pereira, Suzana C. Santos, Jefferson Hollanda Véras, Lee Chen-Chen, Rangel Moreira Silva |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Spectrometry Mass Electrospray Ionization DNA Repair DNA damage Myrtaceae Proton Magnetic Resonance Spectroscopy Health Toxicology and Mutagenesis medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ellagitannin Botany Genetics medicine Animals Pedunculagin Medicinal plants Cyclophosphamide Chromatography High Pressure Liquid chemistry.chemical_classification Traditional medicine Mutagenicity Tests Plant Extracts Comet assay 030104 developmental biology chemistry Seeds Micronucleus test 030221 ophthalmology & optometry Tannins Genotoxicity DNA Damage Mutagens Castalagin |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 810:40-47 |
| ISSN: | 1383-5718 |
| Popis: | Ellagitannins are well-known antioxidants in medicinal plants, foods, and edible fruits, particularly in Myrciaria cauliflora (jabuticaba). Thus, this study aimed to evaluate the protective effects of jabuticaba seed extract (JSE) and pedunculagin using in vivo micronucleus test and comet assay in mouse bone marrow cells, in combination with cyclophosphamide (CP), a bioreductive alkylating agent. The ellagitannin composition of JSE was determined by HPLC/PDA, with castalagin, vescalagin, and pedunculagin as the main compounds (124.4, 45.5, and 15.6 mg/g dw, respectively). Results from pre- and co- treatments with JSE or pedunculagin clearly showed their protective action against CP-induced micronuclei and DNA damage. The effects of both tannins in post-treatments with CP suggested they influence DNA repair systems. These findings indicate that JSE and pedunculagin possess chemopreventive as well as DNA repair-inducing properties. |
| Databáze: | OpenAIRE |
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