Isolation of Human Bone Marrow Mesenchymal Stem Cells Using Different Membrane Markers: Comparison of Colony/Cloning Efficiency, Differentiation Potential, and Molecular Profile
| Autor: | Pierre Charbord, Irene Andreakou, Maria-Christina Kastrinaki, Helen A. Papadaki |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Homeobox protein NANOG
Cellular differentiation medicine.medical_treatment Cell Culture Techniques Biomedical Engineering Clone (cell biology) Medicine (miscellaneous) Bone Marrow Cells Receptors Cell Surface Bioengineering Biology Antigens CD medicine Humans RNA Messenger Clonogenic assay Cells Cultured Homeodomain Proteins Tissue Engineering Reverse Transcriptase Polymerase Chain Reaction Growth factor Mesenchymal stem cell Endoglin Nanog Homeobox Protein Cell Differentiation Mesenchymal Stem Cells Molecular biology Phenotype medicine.anatomical_structure Gene Expression Regulation Leukocyte Common Antigens Bone marrow Octamer Transcription Factor-3 |
| Zdroj: | Tissue Engineering Part C: Methods. 14:333-339 |
| ISSN: | 1937-3392 1937-3384 |
| Popis: | Bone marrow (BM) mesenchymal stem cells (MSCs) represent an interesting field of research for their in vitro properties and the in vivo therapeutic applications. In the present study, we compared the clonogenic and differentiation capacity of MSCs present in three BM-derived populations-namely, the CD105(+)/CD45(-) cells, the glycophorin A (GlycoA)(-)/CD45(-) cells, and the BM mononuclear cells (BMMCs)-by growing/expanding clones from single colony-forming unit fibroblasts (CFU-F). We also quantified the Oct-4 and Nanog mRNA in the CD105(+)/CD45(-) and GlycoA(-)/CD45(-) cells to define the fraction containing more immature MSCs. We found that basic-fibroblast growth factor (bFGF) favors the long-term survival and growth of the more immature MSCs but has no significant effect on MSC clonogenic potential. CFU-F number and clone recovery were higher in CD105(+)/CD45(-) compared to GlycoA(-)/CD45(-) (p < 0.0001 and p = 0.0364, respectively) cells or BMMCs (p < 0.0001 and p = 0.0007, respectively). The relative mRNA expression of Oct-4 and Nanog was significantly increased in CD105(+)/CD45(-) compared to GlycoA(-)/CD45(-) cells (p < 0.0001 and p < 0.0001, respectively). No significant difference was found in the immunophenotypic characteristics and differentiation potential of clones derived from all three cellular sources. These data suggest that the CD105(+)/CD45(-) BM cell fraction is enriched in immature MSCs and, accordingly, represents an appropriate source for MSC culture initiation. |
| Databáze: | OpenAIRE |
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