Emergence of livestock-associated MRSA isolated from cystic fibrosis patients: Result of a Belgian national survey

Autor: Petra Schelstraete, Anne Malfroot, H. Franckx, Olivier Denis, Denis Pierard, Maria Angeles Argudín, Christiane Knoop, V. Y. Miendje Deyi, Magali Dodémont, Laurence Hanssens, Eef Vanderhelst, I. Leroux-Roels, Claire Nonhoff, Ariane Deplano, J. Willekens
Přispěvatelé: Growth and Development, Faculty of Medicine and Pharmacy, Physiotherapy, Human Physiology and Anatomy, Clinical sciences, Pneumology, Supporting clinical sciences, Microbiology and Infection Control, Clinical Biology, Pediatrics, Vriendenkring VUB
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Livestock associated
Cystic Fibrosis
medicine.disease_cause
Animal origin
Cystic fibrosis
0302 clinical medicine
Belgium
Surveys and Questionnaires
Prevalence
Colonization
Prospective Studies
Child
Virulence
Middle Aged
Staphylococcal Infections
Phenotype
Staphylococcus aureus
Child
Preschool
Female
Pneumologie
Pulmonary and Respiratory Medicine
Adult
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
Adolescent
Pédiatrie
Microbial Sensitivity Tests
spa-typing
Microbiology
03 medical and health sciences
Young Adult
medicine
LA-MRSA
Humans
Pediatrics
Perinatology
and Child Health
Aged
Small colony variant
Genetic diversity
business.industry
Infant
Newborn
Infant
biochemical phenomena
metabolism
and nutrition
medicine.disease
bacterial infections and mycoses
030104 developmental biology
030228 respiratory system
CC398
Pediatrics
Perinatology and Child Health
Multilocus sequence typing
business
Follow-Up Studies
Zdroj: Journal of Cystic Fibrosis, 18 (1
ISSN: 1873-5010
Popis: Background: This study aims to determine the prevalence and characteristics of Staphylococcus aureus in Belgian cystic fibrosis (CF) patients. Methods: Non-duplicate respiratory samples from 510 CF-patients (2012−2013) were examined. One isolate per patient was analysed unless different phenotypes were recovered. Isolates were investigated for mecA/mecC, toxins presence, spa-typing, MLST and SCCmec-typing. Potential livestock-associated (LA) isolates were examined for their immune-evasion-cluster (IEC) genes. Results: S. aureus (n = 380), including 41 small-colony variants (SCVs), were isolated from 66.7% patients. The prevalence of methicillin-resistant S. aureus (MRSA) colonization was 4.9%. Two MRSA isolates carried toxic shock syndrome toxin 1 (TSST-1). Most MRSA (65%) belonged to two nosocomial epidemic clones (CC5, CC8) widespread in Belgium. Methicillin susceptible S. aureus (MSSA) showed great genetic diversity. Five of 33 isolates belonging to potential LA-lineages were IEC negative, including three methicillin-resistant isolates, suggesting an animal origin. Conclusions: The MRSA-prevalence in Belgian CF-patients remained constant (2001−2013), but SCV-prevalence increased. Most MRSA belonged to health-care-associated clones. Three patients carrying LA-MRSA were found, requiring further investigation to determine the risk factors for LA-MRSA acquisition.
SCOPUS: ar.j
info:eu-repo/semantics/published
Databáze: OpenAIRE
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