Marginal band microtubules are acetylated by αTAT1
| Autor: | Anne-Sophie Ribba, Laurence Lafanechère, Morgane Batzenschlager, Ekaterina Bourova-Flin, Clotilde Rabat, Karin Sadoul, François Lanza, Saadi Khochbin, Thierry Buchou, Sylvie Moog |
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| Přispěvatelé: | Khochbin, Saadi, Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire [Grenoble] (CHU)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
[SDV]Life Sciences [q-bio] Lysine macromolecular substances 030204 cardiovascular system & hematology microtubules Mice 03 medical and health sciences 0302 clinical medicine Acetyltransferases Microtubule Tubulin Animals Humans Platelet acetylation Tubulin deacetylation biology Chemistry Hematology General Medicine HDAC6 [SDV] Life Sciences [q-bio] 030104 developmental biology acetyltransferase post-translational modification Acetylation Acetyltransferase Biophysics biology.protein |
| Zdroj: | Platelets Platelets, Taylor & Francis, 2020 |
| ISSN: | 0953-7104 1369-1635 |
| Popis: | International audience; The discoid shape of resting platelets is maintained by a peripheral, circular bundle of microtubules called marginal band. Marginal band microtubules are acetylated on lysine 40 of the alpha-tubulin subunits. We have previously shown that the deacetylase HDAC6 is responsible for tubulin deacetylation in platelets and that the hyperacetylated state of the microtubules in HDAC6KO platelets correlates with faster activation/spreading kinetics, pointing to a regulatory role of this modification. So far, the question about the reverse enzyme, responsible for tubulin acetylation in platelets, has remained unanswered. Several enzymes have been described as having tubulin acetylation activity. Here we identify αTAT1 as the enzyme responsible for the acetylation of marginal band microtubules. We show that αTAT1 deficiency has only minor consequences for platelet production and function. A residual tubulin acetylation level in αTAT1 deficient platelet lysates suggests the presence of an additional tubulin-acetylating enzyme that is unable to acetylate marginal band microtubules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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