Circulating miR-185-5p as a Potential Biomarker for Arrhythmogenic Right Ventricular Cardiomyopathy
| Autor: | Riccardo Bariani, Domenico Corrado, Luciano Daliento, Ke Xiao, Indira G. C. Vonhögen, Chiara Romualdi, Barbara Bauce, Libero Vitiello, Leon J. De Windt, Alessandra Rampazzo, Marzia De Bortoli, Alessandra Lorenzon, Thomas Thum, Giulia Poloni, Robin M. W. Colpaert, Martina Calore, Elisa Mazzotti, Claudia Sacchetto, Zenab Mohseni |
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| Přispěvatelé: | Publica, Molecular Genetics, RS: Carim - H05 Gene regulation, Cardiologie, RS: FSE DMG |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty QH301-705.5 heart failure Pilot Projects Disease 030204 cardiovascular system & hematology Sudden death Article Right ventricular cardiomyopathy 03 medical and health sciences 0302 clinical medicine Internal medicine microRNA medicine Humans genetics Biology (General) Arrhythmogenic Right Ventricular Dysplasia 030304 developmental biology arrhythmogenic right ventricular cardiomyopathy 0303 health sciences business.industry MICRORNA Area under the curve biomarkers General Medicine medicine.disease Pathophysiology Circulating MicroRNA MicroRNAs Case-Control Studies Heart failure Cardiology circulating microRNAs Female Cardiomyopathies business |
| Zdroj: | Cells Volume 10 Issue 10 Cells, 10(10):2578. Multidisciplinary Digital Publishing Institute (MDPI) Cells, Vol 10, Iss 2578, p 2578 (2021) |
| ISSN: | 2073-4409 |
| Popis: | Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a genetic cardiac disease characterized by progressive myocardial fibro-fatty replacement, arrhythmias and risk of sudden death. Its diagnosis is challenging and often it is achieved after disease onset or postmortem. In this study, we sought to identify circulating microRNAs (miRNAs) differentially expressed in ARVC patients compared to healthy controls. In the pilot study, we screened the expression of 754 miRNAs from 21 ARVC patients and 20 healthy controls. After filtering the miRNAs considering a log fold-change cut-off of ±1, p-value < 0.05, we selected five candidate miRNAs for a subsequent validation study in which we used TaqMan-based real-time PCR to analyse samples from 37 ARVC patients and 30 healthy controls. We found miR-185-5p significantly upregulated in ARVC patients. Receiver operating characteristic analysis indicated an area under the curve of 0.854, corroborating the link of this miRNA and ARVC pathophysiology. |
| Databáze: | OpenAIRE |
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