Enterobacter Strains Might Promote Colon Cancer
| Autor: | Fikrettin Şahin, Ayten Yazgan-Karataş, Dilsad Yurdakul |
|---|---|
| Přispěvatelé: | Yurdakul, D., Yazgan-Karataş, A., Şahin, Fikrettin, Yeditepe Üniversitesi |
| Rok vydání: | 2014 |
| Předmět: |
Colorectal cancer
Cell Survival Enterobacter Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Applied Microbiology and Biotechnology Microbiology Flow cytometry Cell Line Bacterial Proteins medicine Escherichia coli Humans Viability assay Cell Proliferation Morganella morganii medicine.diagnostic_test Gene Expression Profiling Enterobacteriaceae Infections NF-kappa B Cancer CD24 Antigen Epithelial Cells General Medicine biology.organism_classification medicine.disease Flow Cytometry Real-time polymerase chain reaction Proto-Oncogene Proteins c-bcl-2 Colonic Neoplasms |
| Zdroj: | Current microbiology. 71(3) |
| ISSN: | 1432-0991 |
| Popis: | Many studies have been performed to determine the interaction between bacterial species and cancer. However, there has been no attempts to demonstrate a possible relationship between Enterobacter spp. and colon cancer so far. Therefore, in the present study, it is aimed to investigate the effects of Enterobacter strains on colon cancer. Bacterial proteins were isolated from 11 Enterobacter spp., one Morganella morganii, and one Escherichia coli strains, and applied onto NCM460 (Incell) and CRL1790 (ATCC) cell lines. Cell viability and proliferation were determined in MTS assay. Flow Cytometry was used to detect CD24 level and apoptosis. Real-Time PCR studies were performed to determine NFKB and Bcl2 expression. Graphpad Software was used for statistical analysis. The results showed that proteins, isolated from the Enterobacter spp., have significantly increased cell viability and proliferation, while decreasing the apoptosis of the cell lines tested. The data in the present study indicated that Enterobacter strains might promote colon cancer. Moreover, Enterobacter spp. could be a clinically important factor for colon cancer initiation and progression. Studies can be extended on animal models in order to develop new strategies for treatment. © 2015, Springer Science+Business Media New York. |
| Databáze: | OpenAIRE |
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