Modification of physicochemical properties of actin filaments suppresses cell fragmentation in the execution phase of staurosporine-induced apoptotic processes
| Autor: | Marcin Kamiński, Makoto Masaoka, Anna Hallmann, Chieko Kurono, Anna Majczak, Tsuyoshi Soji, Takashi Wakabayashi, Edyta Niemczyk, Mariusz Karbowski, Dorota Knap, Jakub Kedzior |
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| Rok vydání: | 2004 |
| Předmět: |
Poly ADP ribose polymerase
Population Cell Immunoblotting Antineoplastic Agents Apoptosis Biology Membrane Potentials Cell Line Tumor Depsipeptides medicine Staurosporine Humans Fragmentation (cell biology) Enzyme Inhibitors education Instrumentation education.field_of_study Microscopy Confocal Cytochrome c Actin cytoskeleton Bridged Bicyclo Compounds Heterocyclic Cell biology Mitochondria Actin Cytoskeleton Thiazoles medicine.anatomical_structure Caspases biology.protein Thiazolidines medicine.drug |
| Zdroj: | Journal of electron microscopy. 53(6) |
| ISSN: | 0022-0744 |
| Popis: | Effects of jasplakinolide (JSP), a stabilizer of F-actin, and latrunculin A (LTA), a destabilizer of F-actin, on a series of events occurring in the execution phase of staurosporine (STS)-induced apoptotic processes were studied using human osteosarcoma 143B cells. Time-dependent apparent increases of the population of cells with collapsed membrane potential of mitochondria (Delta Psi(m)) caused by STS treatment were not due to actual decreases in the Delta Psi(m) per cell, but due to the fragmentation of cells resulting in decreases in the number of active mitochondria per cell. Decreases in the Delta Psi(m) in fragmented cells occurred late in the execution phase. Both JSP and LAT failed to prevent STS-induced release of cytochrome c from mitochondria followed by the activation of caspases 3 and 9, the cleavage of poly (ADP-ribose) polymerase (PARP) and apoptotic nuclear fragmentation. However, both drugs prevented STS-induced apoptotic cell fragmentation and decreases in the Delta Psi(m). These results indicate that physicochemical states of actin filaments play a certain role in the execution phase of STS-induced apoptotic processes. |
| Databáze: | OpenAIRE |
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