Targeting of porous hybrid silica nanoparticles to cancer cells
| Autor: | John E. Eriksson, Jessica M. Rosenholm, Cecilia Sahlgren, Mika Lindén, Rasmus Niemi, Emilia Peuhu, Annika Meinander |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Materials science
Polymers media_common.quotation_subject Cell education General Physics and Astronomy Nanoparticle Receptors Cell Surface SDG 3 – Goede gezondheid en welzijn Flow cytometry Drug Delivery Systems Folic Acid SDG 3 - Good Health and Well-being Neoplasms medicine Humans Nanotechnology Polyethyleneimine General Materials Science Receptor Internalization media_common medicine.diagnostic_test Folate Receptors GPI-Anchored General Engineering Mesoporous silica Ligand (biochemistry) Flow Cytometry Silicon Dioxide Molecular biology Coculture Techniques medicine.anatomical_structure Microscopy Fluorescence Cancer cell Biophysics Nanoparticles Carrier Proteins Porosity HeLa Cells |
| Zdroj: | ACS Nano, 3(1), 197-206. American Chemical Society |
| ISSN: | 1936-0851 |
| Popis: | Mesoporous silica nanoparticles functionalized by surface hyperbranching polymerization of polyethylene imine), PEI, were further modified by introducing both fluorescent and targeting moieties, with the aim of specifically targeting cancer cells. Owing to the high abundance of folate receptors in many cancer cells as compared to normal cells, folic acid was used as the targeting ligand. The internalization of the particles in cell lines expressing different levels of folate receptors was studied. Flow cytometry was used to quantify the mean number of nanoparticles internalized per cell. Five times more particles were internalized by cancer cells expressing folate receptors as compared to the normal cells expressing low levels of the receptor. Not only the number of nanoparticles internalized per cell, but also the fraction of cells that had internalized nanoparticles was higher. The total number of particles internalized by the cancer cells was, therefore, about an order of magnitude higher than the total number of particles internalized by normal cells, a difference high enough to be of significant biological importance. In addition, the biospecifkally tagged hybrid PEI-silica particles were shown to be noncytotoxic and able to specifically target folate receptor-expressing cancer cells also under coculture conditions. © 2009 American Chemical Society. |
| Databáze: | OpenAIRE |
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